不同辣椒材料对疫病的抗性研究

doi:10.11924/j.issn.1000-6850.casb20200100010

摘要/Abstract

摘要：

为筛选抗性种质资源,进行抗性品种选育和推广种植,利用苗期游动孢子灌根法对206份江西省地方辣椒材料进行抗病性鉴定,并初步分析不同时期材料的发病情况。研究结果表明,不同材料间的抗病性存在较为明显的差异,无论是病株率还是病情指数均有显著差异性,病株率在0~30.0%、30.1%~70.0%、70.1%~100%之间的材料分别为21、32、153份,占供试材料的10.19%、15.53%、74.27%;表现为高抗、抗病、中抗和感病的材料分别有15、17、23、151份,占供试材料的7.28%、8.25%、11.17%和73.30%;鉴定出的‘赣椒18号’、‘赣椒35号’、C047、WY38、CM334和A204等15份高抗材料是很好的抗疫病资源。分析供试材料在2次调查结果中的发病情况可知,材料间的发病速度（侵入和扩展）存在显著差异性。

关键词: 辣椒, 疫霉菌, 病情指数, 抗性, 鉴定

Abstract:

To screen resistant germplasm resources for the selection and promotion of resistant pepper cultivars, the resistance of 206 local pepper cultivars in Jiangxi was identified by drenching them with zoosperm, and the differences of resistance in different periods were preliminarily analyzed. The results showed that both the disease rate and disease index varied widely among different materials. There were 21, 32 and 153 materials with the disease rate of 0-30.0%, 30.1%-70.0% and 70.1%-100%, respectively, accounting for 10.19%, 15.53% and 74.27% of the tested materials. There were 15, 17, 23 and 151 cultivars with high resistance, resistance, moderate resistance and susceptibility, respectively, accounting for 7.28%, 8.25%, 11.17% and 73.30% of the tested materials. 15 materials, such as ‘Ganjiao 18’, ‘Ganjiao 35’, C047, WY38, CM334, A204 and so on, were identified with high resistance. According to the analysis of the incidence, there was a significant difference of the incidence rate (invasion and spread) among the materials.

Key words: pepper, Phytophthora capsici, disease index, resistance, identify

中图分类号:

S432.4+4

何烈干, 邹芬, 胡铭, 熊水平, 马辉刚. 不同辣椒材料对疫病的抗性研究[J]. 中国农学通报, 2020, 36(34): 110-116.

He Liegan, Zou Fen, Hu Ming, Xiong Shuiping, Ma Huigang. Resistance of Pepper Cultivars to Phytophthora capsici[J]. Chinese Agricultural Science Bulletin, 2020, 36(34): 110-116.

图/表 3

参考文献 34

[1]	易图永, 谢丙炎, 张宝玺, 等. 辣椒疫病防治研究进展[J]. 中国蔬菜, 2002,1(5):52-55.
[2]	Oelke L M, Bosland P W, Steiner R. Differentiation of race specific resistance to phytophthora root rot and foliar blight in Capsicum annuum[J]. Journal of the American Society for Horticultural Science, 2003,128(2):213-218. doi: 10.21273/JASHS.128.2.0213 URL
[3]	Hausbeck M K, Lamour K H. Phytophthora capsici on vegetable crops: research progress and management challenges[J]. Plant Dis, 2004,88(12):1292-1303. doi: 10.1094/PDIS.2004.88.12.1292 URL pmid: 30795189
[4]	姜国庆, 丁和明. 辣椒疫病的发生及防治技术[J].湖北植保, 2014(1):26,28.
[5]	Kamoun S, Furzer O, Jones J D, et al. The top 10 oomycete pathogens in molecular plant pathology[J]. Molecular plant pathology, 2015,16(4):413-434. doi: 10.1111/mpp.12190 URL pmid: 25178392
[6]	王秋月, 马冠华, 尹学伟, 等. 重庆地区辣椒疫霉对氟吡菌胺的敏感性及抗性突变体的生物学性状[J]. 农药学学报, 2019,21(2):165-174.
[7]	Gevens A J, Donahoo R S, Lamour K H, et al. Characterization of Phytophthora capsici causing foliar and pod blight of snap bean in Michigan[J]. Plant Disease, 2008,92(2):201-209. doi: 10.1094/PDIS-92-2-0201 URL pmid: 30769389
[8]	Granke L L, Quesada-Ocampo L, Lamour K, et al. Advances in research on Phytophthora capsici on vegetable crops in the United States[J]. Plant Disease, 2012,96(11):1588-1600. doi: 10.1094/PDIS-02-12-0211-FE URL pmid: 30727465
[9]	雷阳, 成妍, 乔宁, 等. 辣椒苗期抗感疫病比较转录组学分析[J]. 华北农学报, 2019,34(3):194-202.
[10]	Leonian L H. Stem and fruit blight of peppers caused by Phytophthora capsici sp. nov.[J]. Phytopathology, 1922,12(9):401-408.
[11]	Lamourd K H, Stam R, Jupe J, et al. The oomycete broad-host-range pathogen Phytophthora capsici[J]. Molecular Plant Pathology, 2012(13):329-337. doi: 10.1111/j.1364-3703.2011.00754.x URL
[12]	韩秀英, 赵卫松, 齐永志, 等. 辣椒疫霉对双炔酰菌胺敏感性及对其他杀菌剂的交互抗性[J]. 植物保护学报, 2011,38(2):173-177.
[13]	秦维彩, 闫晓静, 檀根甲, 等. 烯酰吗啉与嘧菌酯对辣椒疫霉病菌生物活性的比较[J]. 植物保护学报, 2010,37(1):42-48.
[14]	戚仁德, 丁建成, 高智谋, 等. 安徽省辣椒疫霉对甲霜灵的抗药性监测[J]. 植物保护学报, 2008,35(3):245-250.
[15]	Bartlett D W, Clough J M, Gonwin J R, et al. The strobilurin fungicides[J]. Pest Management Science, 2002,58:649-662. doi: 10.1002/ps.520 URL pmid: 12146165
[16]	汪涛, 迟元凯, 赵伟, 等. 多粘类芽孢杆菌TC35的鉴定及对辣椒疫病的田间防效[J]. 安徽农业科学, 2018,46(4):138-139.
[17]	李智军, 龙卫平, 郑锦荣, 等. 2个辣椒疫病抗性资源的抗性遗传分析[J]. 华南农业大学学报, 2008,29(2):30-33.
[18]	Bonnie R G, Ebenezer A O, Gurmel S S. A differential series of pepper(Capsicum annuum) lines delineates fourteen physiological races Phytophora capsici[J]. Euphytica, 2008,162:23-30.
[19]	谭清群, 袁洁, 杨学辉, 等. 贵州省辣椒新品种对疫病和青枯病的抗性鉴定研究[J]. 种子, 2014,33(11):82-85.
[20]	赫卫, 张慧, 王莹. 辣椒对疫霉抗性的快速鉴定[J]. 植物保护, 2018,44(2):145-148.
[21]	林柏青, 张松林. 辣椒品种抗疫病鉴定方法的初步研究[J].中国蔬菜,1994(4):21-24.
[22]	王述彬, 袁希汉, 邹学校. 中国辣椒优异种质资源评价[J]. 江苏农业学报, 2001,17(4):244-247.
[23]	李屹, 田晓丽. 辣椒资源材料抗疫病鉴定及主要农艺性状评价[J].北方园艺,2012(14):138-141.
[24]	王飞, 姚明华, 焦春海. 泰国辣椒种质资源鉴定与评价[J]. 湖北农业科学, 2010,49(9):2146-2148.
[25]	谭清群, 袁洁, 杨学辉, 等. 贵州省辣椒新品种对疫病和青枯病的抗性鉴定研究[J]. 种子, 2014,33(11):82-85.
[26]	Candole B L, Conner P J, Ji P. Screening Capsicum annuum accessions for resistance to six isolates of Phytophthora capsici[J]. Hort Science, 2010,45(2):254-259.
[27]	乔宁, 马蓉丽, 王瑞丽, 等. 辣椒疫病的发生及抗性研究进展[J]. 山西农业科学, 2016,44(6):885-888.
[28]	毛爱军, 胡治, 俞世敏, 等. 辣(甜)椒疫病菌致病力及接种浓度[J]. 北京农业科学, 1997,15(1):35-37.
[29]	杨学辉, 袁洁, 谢海呈. 贵州省辣椒主栽品种抗疫病性鉴定[J]. 西南农业学报, 2006,18(6):791-793.
[30]	吴石平, 杨学辉, 何海永, 等. 贵州部分辣椒品种对疫病的抗性鉴定[J].贵州农业科学,2010(2):89-90.
[31]	蓬桂华, 邢丹, 牟玉梅, 等. 贵州地方辣椒抗疫病性鉴定[J].现代农业科技,2015(20):64-65,73.
[32]	韩帅, 张河庆, 吴婕, 等. 四川主要辣椒栽培品种对疫病的抗性鉴定及评价[J].中国蔬菜,2019(7):56-61.
[33]	马辉刚, 何烈干, 陈学军, 等. 两种方法鉴定辣椒疫病抗性研究[J]. 江西农业大学学报, 2013,35(3):536-542.
[34]	何烈干, 周银生, 陈学军, 等. 辣椒种质资源抗疫病性及发病速度分析[J]. 江西农业大学学报, 2017,39(4):691-698.