关键词: 快繁, 快繁

Abstract:

In order to study the miRNAs information and miRNA expressions under iron-deficiency stress of Fe-efficient plant Malus xiaojinensis, to further understand the iron deficiency molecules regulatory mechanism of the Malus xiaojinensis. In this study, miR394a was isolated from total RNA of Malus xiaojinensis which was extracted by the modified CTAB method as a template. miR394a expression pattern was detection by real-time PCR and the target genes and their functions of miR394a were also predicted through the bioinformatics methods. The miR394a was highly conservative which had high homology with other species. miR394a was expressed both in root and leaf under iron deficiency, but the express pattern not the same. miR394 had a quick response in roots, its up regulation was detected after one day of iron deficiency. It had a relatively slow response in leaves, whose up-regulation was detected after three days of iron deficiency. miR394a target genes prediction showed that miR394a was mainly regulation the transcription factors and genes which were participated in metabolic pathways. The results showed that miR394a was induced by iron deficiency stress and played an important role in iron deficiency regulation for involved in the iron deficiency regulatory pathways of Malus xiaojinensis.