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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (10): 129-132.doi: 10.11924/j.issn.1000-6850.casb14110160

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

4个新品种玉簪愈伤组织的诱导及组培扩繁研究

胡岷桐1,王克凤2,丁 言1,顾德峰1   

  1. (1吉林农业大学园艺学院,长春 130118；2长春科技学院,长春 130600）
  • 收稿日期:2014-11-26 修回日期:2015-03-06 接受日期:2015-02-10 出版日期:2015-05-05 发布日期:2015-05-05
  • 通讯作者: 顾德峰
  • 基金资助:
    吉林省科技发展项目(20110262)。

Study on Callus Induction and Tissue Culture Propagation of Four New Varieties of Hosta

Hu Mintong1, Wang Kefeng2, Ding Yan1, Gu Defeng1   

  1. (1College of Horticulture, Jilin Agricultural University, Changchun 130118; 2Changchun Institute of Technology, Changchun 130600)
  • Received:2014-11-26 Revised:2015-03-06 Accepted:2015-02-10 Online:2015-05-05 Published:2015-05-05

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摘要： 为建立4个玉簪新品种的组培再生体系，以MS为基本培养基，添加不同质量浓度6-BA和2,4-D，研究其外植体组培苗的愈伤组织诱导以及继代扩繁的最适生长培养基。愈伤组织的诱导中，高浓度的6-BA(≥3.0mg/L)与低浓度的2,4-D(≤0.1mg/L)配比有利于愈伤组织的生成。MS+1.0mg/L6-BA+0.3mg/LNAA+35g/L蔗糖+12g/L琼脂的培养基可以使HostaBigDaddy（编号H1）和‘金鹰’（编号H2）的增殖倍数分别达2.54和2.78，而MS+1.0mg/L6-BA+0.2mg/LNAA+30g/L蔗糖+11g/L琼脂可以使‘小黄金叶’（编号H6）的增殖倍数达3.01，而最适宜H15增殖的培养基则是MS+3.0mg/L6-BA+0.1mg/LNAA+30g/L蔗糖+12g/L琼脂。研究结果为4个新品种玉簪愈伤组织的诱导及组培扩繁研究提供了有利数据依据，在此方法下愈伤组织诱导率最大，并且扩繁增殖倍数最大。

关键词: 甘薯, 甘薯, 品种, 产量, 淀粉, RVA糊化特性

Abstract: For the establishment of tissue culture regeneration system of four new varieties of Hosta, we chose MS as the basic culture medium, and added in different concentrations of 6-BA and NAA to study the optimum growth medium of callus induction and subculture propagation of explant tissue culture. The results showed that high concentrations of 6- BA (greater than or equal to 3.0 mg/L) with 2,4- D (greater than or equal to 0.1 mg/L) were conducive to generate callus about the callus induction. MS+ 1.0 mg/L 6-BA+ 0.3 mg/L NAA+ 35 g/L sucrose + 12 g/L agar could make the proliferation multiple of Hosta Big Daddy (No. H1) and the ‘Eagle’(No. H2) up to 2.54 and 2.78, and the MS+ 1.0 mg/L 6-BA+ 0.2 mg/L NAA+30 g/L sucrose+11 g/L agar could make the‘little Huang Jinye’(No. H6) proliferation multiple up to 3.01, and the most suitable medium on proliferation of H15 was MS+ 3.0 mg/L 6-BA+ 0.1 mg/L NAA+ 30 g/L sucrose+ 12 g/L agar. The study results can provide favorable data for the induction and tissue culture of four new varieties of Hosta callus, callus induction rate of this method is the maximum, and the proliferation multiple of propagation is also the maximum.