欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (28): 163-168.

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

不同表面消毒方法对核桃叶内生菌分离效果的比较

刘丽   

  • 收稿日期:2012-04-12 修回日期:2012-05-23 出版日期:2012-10-05 发布日期:2012-10-05

Comparison of different surface disinfection methods for the isolation of the endophytes in walnut leaves

  • Received:2012-04-12 Revised:2012-05-23 Online:2012-10-05 Published:2012-10-05

PDF (PC)

1058

摘要：

摘 要：【研究目的】表面消毒是植物内生菌分离的关键步骤之一，不同的表面消毒方法不仅会影响表面消毒效果，对内生菌的分离效果也有一定影响。本文分析比较了几种不同的表面消毒方法下分离获得的内生菌数量及种类。【方法】采用传统的分离方法，即真菌用组织块法，细菌用稀释梯度法进行分离。【结果】结果表明无论是内生真菌还是内生细菌二氧化氯方法处理下获得的内生菌种类最多。即二氧化氯法获真菌6株，鉴定为5个属，细菌37株，鉴定为11个属种（其中包括1个放线菌属）；次氯酸钠法获真菌8株，鉴定为3个属，细菌4株，鉴定为3个属种；升汞法获真菌3株，鉴定为3个属，细菌1株，为未知种。从分离内生菌的数量上比较，二氧化氯方法分离获得的细菌数显著性的多于其他两种方法，由多到少依次为二氧化氯法、次氯酸钠法、升汞法。【结论】二氧化氯表面消毒更适合进行内生菌多样性研究，但不同的表面消毒方法会获得不同的机会菌株。

关键词: 产量, 产量

Abstract:

Abstract：【OBJECTIVE】The surface disinfection is one of the critical steps during isolating the plant endophytes. The purpose of this article was to analyze and compare the affection of disinfection to the species and quantity of the endophytes isolated from walnut leaves. 【METHOD】The fungi were identified by the tissue isolation，the bacteria were identified by the dilution method.【RESULTS】The results indicated that the immersing 30S in 0.3% ClO2 was the best for isolating endophytic in walnut leaves, namely, five genera fungi and eleven species bacteria (including one actinobacteria) was isolated by ClO2, Three genera fungi and three species bacteria were isolated by NaClO, Three genera fungi and one specie bacteria were isolated by HgCl. 【CONCLUSION】This study also indicated that different disinfectants would isolate different endophytic groups. Anyone can not instead of others.