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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (36): 237-240.

• 生物技术科学 • 上一篇    下一篇

桑花叶型萎缩病病原的检测与序列分析

孔卫青 杨金宏   

  • 收稿日期:2012-05-22 修回日期:2012-07-22 出版日期:2012-12-25 发布日期:2012-12-25
  • 基金资助:

    陕西省科技厅农业攻关项目;陕西省教育厅重点实验室项目

The Detection and Sequence Analysis of Pathogen from Mulberry Mosaic Dwarf Disease

  • Received:2012-05-22 Revised:2012-07-22 Online:2012-12-25 Published:2012-12-25

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摘要：

旨在获得安康地区桑花叶萎缩病病原序列，为桑花叶萎缩病的防治提供更多的信息。用RT-PCR技术对安康地区染花叶型萎缩病病株的病原进行了分离，克隆测序并在Mfold网站预测其二级结构。该病的病原为桑花叶萎缩类病毒MMDVd-201110151（GenBank登录号：JQ809700），核苷酸长度357 bp，GC含量50.7%，219 bp后38个碱基与樱桃小圆形类病毒样核糖核酸具有约85%的一致性。Mfold网站预测二级结构有58.3%的碱基配对，两端为分枝状结构。成功分离到安康地区桑花叶型萎缩病病原。

关键词: 产量, 产量

Abstract:

The aims were to obtain the pathogenic sequence of mulberry mosaic dwarf disease in Ankang area, and provide more information on the control of the disease. The pathogen of mulberry mosaic dwarf disease in Ankang area was isolated by RT-PCR, and the secondary structure was predicted in Mfold web. The length of the pathogen, MMDVd-201110151 (GenBank accession number: JQ809700), was 357 bp, and the GC percent was 50.7% in it. The 38 bp behind 219 bp had 85% identity with cscRNA1.150. The secondary structure of MMDVd-201110151 was predicted in Mfold web, 58.3% pairs in the secondary structure, and the two ends in it were both branched. The pathogen of mulberry mosaic dwarf disease in Ankang area was isolated successfully.

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