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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (26): 63-66.

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

应用荧光定量PCR对细粒棘球绦虫miRNAs在虫体不同发育阶段表达量的研究

郝力力 李必富   

  • 收稿日期:2012-06-01 修回日期:2012-06-14 出版日期:2012-09-15 发布日期:2012-09-15
  • 基金资助:

    西南民族大学 中央高校基本科研业务费

Quantification Analysis of miRNAs Expression Level in Different Developing Stages of Echinococcus granulosus Using Real-time PCR

  • Received:2012-06-01 Revised:2012-06-14 Online:2012-09-15 Published:2012-09-15

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摘要：

为确定通过Solexa高通量测序鉴定出的大量细粒棘球绦虫miRNAs序列在不同生活史阶段的表达量。采用SYBR染料法和Race试剂盒相结合的荧光定量方法对原头节和生发层阶段的miRNAs表达进行了检测分析。结果表明：两阶段miRNAs表达差异较大，其中mir-71、mir-7、mir-1和mir-19在生发层阶段表达量较高，而let-7则在原头节阶段表达较高。说明这些miRNAs可能在细粒棘球绦虫发育阶段的基因转录调控中起着重要作用。

关键词: 江西省, 江西省

Abstract:

in this experiment, many new miRNAs in Echinococcus granulosus were discovered by high-throughout sequencing technology Solexa. To investigate the expression level of these miRNAs in different life stages of Echinococcus granulosus, Real-time PCR was used to relatively quantify the dominantly expressed miRNAs in protoscolex and germinal layer by combination of SYBR Green PCR and Race kit. It was found that mir-71, mir-7, mir-1and mir-19 were highly expressed at germinal layer other than protoscolex and let-7 was more aboudunt in protoscolex than germinal layer. The results suggested that miNRAs might play an important role in regulation of genes transcription in different developing stages of Echinococcus granulosus.