关键词: 原核表达, 原核表达

Abstract: Abstract: 【Objective】 This study aimed to reveal the influence of CHA SX-1 to parents, traits in Brassica napus.L, and explore the standardized scientific method of using CHA SX-1 in hybrids production in B. napus L. 【Method】the two tested varieties of B. napus in the field was sprayed SX-1 with 5.5mg/L on leaves for the first time，when the height of female parent stem was about 20cm, and the biggest bud of 80% plants reached to about 2mm, and pollen development was in uninucleate stage to the late uninucleate stage. After 7 to 10 days , SX-1 with 6.5mg/L was sprayed for the second time with2.5ml per plant .【Result】After spraying SX-1,sterility of the two female parents was completely, and the sterility rate reached to 99%,and self-cross of the two female parents could not obtain seeds, but outcrossing might set seeds normally, and plants did not show obvious phytotoxicity. However, parts of organ morphology and agronomic traits changed significanclly, For example, plant height of female parents and effective branch position lowered, and main inflorescences shortened, buds, sepals and petals became smaller, filaments shortened and its weight lightened, and pistil elongated and its weight increased, and pods and 1000-seeds weight increased, seeds per silique reduced, but the yield of hybrids production just reduced 9.07% and 15.27% than CK, and correlated traits and yield traits of the two male parents did not been influenced significantly.【Conclusion】SX-1 is a kind of efficient CHA for rape, and it is also safe and feasible in hybrids production of B. napus L.