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中国农学通报 ›› 2016, Vol. 32 ›› Issue (1): 22-27.doi: 10.11924/j.issn.1000-6850.casb15100075

• 林学 园艺 园林 • 上一篇    下一篇

紫薇优良品种‘晓明1号’组培快繁体系的建立

蔡 能,王晓明,李永欣,曾慧杰,乔中全   

  1. 湖南省林业科学院,湖南省林业科学院,湖南省林业科学院,湖南省林业科学院,湖南省林业科学院
  • 收稿日期:2015-10-20 修回日期:2015-12-15 接受日期:2015-12-01 出版日期:2016-01-28 发布日期:2016-01-28
  • 通讯作者: 蔡 能
  • 基金资助:
    长沙市科技计划项目“大红高抗紫薇新品种高效繁育技术产业化开发”(K1406216-21)。

Establishment of a Tissue Culture and Rapid Propagation System of Lagerstroemia indica‘Xiaoming 1’

  • Received:2015-10-20 Revised:2015-12-15 Accepted:2015-12-01 Online:2016-01-28 Published:2016-01-28

摘要: 建立紫薇优良品种‘晓明1 号’组培快繁体系,为大规模繁殖提供技术。以带腋芽茎段为外植体,对组培快繁体系中的基本培养基、生长调节剂浓度、组培苗移栽进行了研究,建立组培快繁体系。结果表明:带腋芽茎段接种在DKW+ BA 1.0 mg/L+ NAA 0.05 mg/L+ PVP 300 mg/L的初代培养基上,腋芽萌发率95.56%,植株生长健壮;‘晓明1 号’紫薇增殖培养及生根培养最佳配方为DKW+ BA 1.0 mg/L+IBA 0.05 mg/L和1/2DKW+ NAA 0.1 mg/L+ AC 300 mg/L,增殖系数达到5.08,生根率达到100%,平均根条数6.2 条,平均根长4.12 cm;用泥炭土:珍珠岩为2?1 或3?1 作为基质,以营养钵作为容器,组培苗移栽成活率达到95.33%以上,组培苗叶色鲜艳,长势良好。该研究获得了适合‘晓明1 号’紫薇的组培方法,为品种的大规模繁殖和大面积推广提供了参考。

关键词: 陆稻, 陆稻, 干旱胁迫, 聚乙二醇, 生理反应

Abstract: In order to establish a tissue culture and rapid propagation system, taking stems with axillary buds of Lagerstroemia indica‘Xiaoming 1’as testing materials, basic medium, concentrations of plant growth regulators, proliferation and rooting stage, and the transplanting method were studied. The results showed that the medium DKW BA 1.0 mg/L NAA 0.05 mg/L PVP 300 mg/L was suitable for inducing germination of stem auxiliary buds, and the rate of germination was up to 95.56%. The best proliferation medium was DKW BA 1.0 mg/L IBA 0.05 mg/L, and the multiplication coefficient was 5.08 with strong plantlets. The rooting percentage, average root number and length per shoot reached 100%, 6.2 and 4.12 cm, respectively, on the medium 1/2DKW NAA 0.1 mg/L AC 300 mg/L. Taking nutrition pot as container, peat soil and perlite as transplanting medium (3:1 or 2:1), the transplant survival rate was over 95.33%. There were many lateral roots and the plantlets which grew rapidly and vigorously. This method was suitable for tissue culture of L. indica ‘Xiaoming 1’and laid foundations for its large-scaled propagation and large area promotion.

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