转抗旱基因棉对土壤酶活性及速效养分含量的影响

doi:10.11924/j.issn.1000-6850.casb16010131

中国农学通报 ›› 2016, Vol. 32 ›› Issue (12): 77-83.doi: 10.11924/j.issn.1000-6850.casb16010131

所属专题：生物技术；棉花

转抗旱基因棉对土壤酶活性及速效养分含量的影响

赵孝丹^1,2,赵建宁²,红雨¹,李刚²,修伟明²,雒珺瑜³,杨殿林²

(¹内蒙古师范大学生命科学与技术学院,呼和浩特 010022；²农业部环境保护科研监测所,天津 300191；³中国农业科学院棉花研究所,河南安阳 455000）

收稿日期:2016-01-26 修回日期:2016-04-13 接受日期:2016-03-18 出版日期:2016-04-27 发布日期:2016-04-27
通讯作者: 红雨
基金资助:
转基因生物新品种培育重大专项“转基因棉花环境安全评价技术-复合性状转基因棉花对土壤生态环境安全评价技术研究”(2014ZX08011-002)；国家自然科学基金“转Bt基因棉花外源重组DNA土壤分布特征与水平转移研究”(31200424)。

Effects of Transgenic Drought-resistant Cotton on Enzyme Activities and Available Nutrients Content in Soil

Zhao Xiaodan^1,2, Zhao Jianning², Hong Yu¹, Li Gang², Xiu Weiming², Luo Junyu³, Yang Dianlin²

(¹College of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010022;²Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191; ³Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang Henan 455000)

Received:2016-01-26 Revised:2016-04-13 Accepted:2016-03-18 Online:2016-04-27 Published:2016-04-27

摘要/Abstract

摘要： 旨在探究不同生育期内种植转抗旱基因棉及其常规棉对土壤酶活性及速效养分含量的影响。应用化学分析法和溶剂浸提法对2种棉花的土壤酶活性和速效养分含量进行比较分析。结果表明，在苗期，转抗旱基因棉土壤速效磷含量显著低于其常规棉，而土壤铵态氮、硝态氮含量和脲酶活性则显著高于其常规棉，其余4个生育期它们的含量与活性均无显著差异，土壤碱性磷酸酶和过氧化氢酶活性5个生育期差异均不显著。对土壤酶活性和土壤速效养分含量的相关性分析表明，土壤铵态氮含量与土壤脲酶活性呈显著正相关，而与土壤碱性磷酸酶活性呈显著负相关；土壤速效磷与土壤全氮呈极显著负相关；土壤硝态氮含量与土壤碱性磷酸酶活性呈显著负相关，与土壤铵态氮含量显著正相关。综上，转抗旱基因棉花的种植几乎并不影响土壤速效养分含量及土壤酶活性，但随着生育期的不同其土壤速效养分含量及土壤酶活性也不同。

关键词: 玉米, 玉米, 耐酸铝, 耐低磷, 基因型差异

Abstract: The objective of this study is to investigate the effect of planting drought-resistant transgenic cotton and conventional cotton during different growth stages on soil enzyme activities and available nutrient content. By applying the method of chemical analysis and solvent extraction method, the soil enzyme activities and available nutrient content of the two kinds of cotton were compared and analyzed. Results demonstrated that at seedling stage, soil available phosphorus content of drought-resistant transgenic cotton was significantly lower than that of conventional cotton, soil ammonium nitrogen and nitrate nitrogen content and urease activity of drought-resistant transgenic cotton were markedly higher as compared with that of conventional cotton, while at other 4 growth stages, no evident difference was found in their contents and activities; soil alkaline phosphatase and catalase activities showed no obvious difference in 5 different growth stages. The correlation analysis of soil enzyme activities and available nutrient content revealed that soil ammonium nitrogen content was significantly and positively correlated with soil urease activity, while it was significantly and negatively correlated with soil alkaline phosphatase activity; soil available phosphorus and soil total nitrogen presented an extremely and significantly negative correlation; soil nitrate nitrogen content showed a significantly negative correlation with soil alkaline phosphatase activity while a significantly positive correlation with soil ammonium nitrogen content. In conclusion, planting drought-resistant transgenic cotton did not affect the available nutrient content and soil enzyme activity. However, the available nutrient content and soil enzyme activity were different in different growth stages of cotton.

赵孝丹,赵建宁,红雨,李刚,修伟明,雒珺瑜,杨殿林. 转抗旱基因棉对土壤酶活性及速效养分含量的影响[J]. 中国农学通报, 2016, 32(12): 77-83.

Zhao Xiaodan,Zhao Jianning,Hong Yu,Li Gang,Xiu Weiming,Luo Junyu and Yang Dianlin. Effects of Transgenic Drought-resistant Cotton on Enzyme Activities and Available Nutrients Content in Soil[J]. Chinese Agricultural Science Bulletin, 2016, 32(12): 77-83.

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转抗旱基因棉对土壤酶活性及速效养分含量的影响

Effects of Transgenic Drought-resistant Cotton on Enzyme Activities and Available Nutrients Content in Soil

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