乳酸高产乳酸菌株的分离与筛选

doi:10.11924/j.issn.1000-6850.casb16040180

中国农学通报 ›› 2017, Vol. 33 ›› Issue (7): 41-47.doi: 10.11924/j.issn.1000-6850.casb16040180

乳酸高产乳酸菌株的分离与筛选

平文祥,杨睿睿,杜仁鹏,王瑶,王琪,赵丹

黑龙江大学生命科学学院微生物省高校重点实验室;黑龙江大学农业微生物技术教育部工程研究中心,黑龙江大学生命科学学院微生物省高校重点实验室,黑龙江大学生命科学学院微生物省高校重点实验室,黑龙江大学生命科学学院微生物省高校重点实验室,黑龙江大学生命科学学院微生物省高校重点实验室,黑龙江大学生命科学学院微生物省高校重点实验室

收稿日期:2016-04-29 修回日期:2017-01-18 接受日期:2016-07-25 出版日期:2017-03-16 发布日期:2017-03-16
通讯作者: 赵丹
基金资助:
国家自然科学基金青年科学“酸菜发酵生态系统中细菌群落与代谢物组特征耦合机制”(31300355)；哈尔滨市科技局青年后备人才项目“功能性乳酸菌发酵酸菜生态学过程研究”(2014RFQXJ101)；黑龙江大学研究生创新科研项目资金“酸菜发酵生态系统中细菌群落与代谢物组特征耦合机制”(YJSCX2015-090HLJU)。

The Insolationan and Screening of A High Lactate-producing Lactic Acid Bacterium

平文祥,,,, and

Received:2016-04-29 Revised:2017-01-18 Accepted:2016-07-25 Online:2017-03-16 Published:2017-03-16

摘要/Abstract

摘要： 乳酸作为一种天然有机酸广泛应用于食品、纺织业、化学和制药工业。本研究从自然酸菜发酵液中分离筛选出一株乳酸高产的乳酸菌11MZ-5-1,发酵72 h时,乳酸含量达24.88±0.21 g/L。利用生理生化及16S rDNA序列分析法对菌株进行鉴定,构建系统发育树,确定种属地位。结果表明,菌株11MZ-5-1的生长温度范围为20-45℃,最适生长温度为30-37℃；NaCl耐受浓度为0-12%,最适生长范围为0-4%；pH耐受范围为3.5-7.5,最适生长pH为5.5-6.5。16S rDNA部分序列长度为1438 bp,与干酪乳杆菌(Lactobacillus casei) str. Zhang相似性最高,达99%。结合形态学观察及理化特性,该菌株鉴定为L. casei。L. casei 11MZ-5-1发酵周期短,产酸量高,在乳酸工业生产领域有广泛的应用前景。

关键词: 糜子, 糜子, 麸皮, 酚酸, 水酶法提取, 响应面分析

Abstract: Lactate is widely used in food, textile, chemical and pharmaceutical industries as a natural organic acid. A high lactate-producing bacterium, strain 11MZ-5-1 was isolated from the liquid sample of pickle Chinese cabbage. The concentration of lactate produced by strain 11MZ-5-1 was 24.88±0.21 g/L at 72 h. The strain was identified according to morphological, physiological and biochemical methods. Meanwhile, the phylogenetic tree was conducted based on its partial 16S rDNA sequence. The results showed that strain 11MZ-5-1 could grow at 20-45℃, with the optimum temperature range 30-37℃. The salt-tolerance range was 0-12%. The tolerance of pH was 3.5-7.5 with the optimum pH was 5.5 to 6.5. Its partial sequence of the 16S rDNA was 1438 bp which was 99% similar with Lactobacillus. casei str. Zhang. Strain 11MZ-5-1 was identified as L. casei. The results suggested its great application potential in industrial production due to the short fermentation period and high lactate production.

中图分类号:

平文祥,杨睿睿,杜仁鹏,王瑶,王琪,赵丹. 乳酸高产乳酸菌株的分离与筛选[J]. 中国农学通报, 2017, 33(7): 41-47.

平文祥,,,, and . The Insolationan and Screening of A High Lactate-producing Lactic Acid Bacterium[J]. Chinese Agricultural Science Bulletin, 2017, 33(7): 41-47.

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乳酸高产乳酸菌株的分离与筛选

The Insolationan and Screening of A High Lactate-producing Lactic Acid Bacterium

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