丹参多倍体开花前后丹酚酸生物合成关键酶活性的变化

doi:10.11924/j.issn.1000-6850.casb2021-0901

中国农学通报 ›› 2022, Vol. 38 ›› Issue (19): 73-76.doi: 10.11924/j.issn.1000-6850.casb2021-0901

所属专题：生物技术；园艺

丹参多倍体开花前后丹酚酸生物合成关键酶活性的变化

吴顺(), 马晓静, 邱云, 孙健鹏, 翟茜, 刘奇

中南林业科技大学生命科学与技术学院,长沙 410018

收稿日期:2021-09-16 修回日期:2022-03-13 出版日期:2022-07-05 发布日期:2022-07-13
作者简介:吴顺,男,1976年出生,湖南宁乡人,副教授,博士,主要从事中药植物种质资源的分子选育与综合利用研究。通信地址：410018 湖南省长沙市韶山南路498号中南林业科技大学生命科学与技术学院,Tel：0731-85623486,E-mail： wushuncn@csuft.edu.cn。
基金资助:
湖南省教育厅科学研究重点项目“不同倍性丹参多倍体的生理特性及遗传变异研究”(20A519);长沙市科技局科技计划项目“丹参新种质的选育及快速繁殖技术研究”(kq2004090);中南林业科技大学教学改革研究项目“‘双一流’背景下研讨式分子生物学实验教学的设计与实践”。

Changes of Key Enzyme Activities for Salvianolic Acid Biosynthesis Before and After Flowering in Salvia miltiorrhiza Polyploid

WU Shun(), MA Xiaojing, QIU Yun, SUN Jianpeng, ZHAI Xi, LIU Qi

School of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410018

Received:2021-09-16 Revised:2022-03-13 Online:2022-07-05 Published:2022-07-13

摘要/Abstract

摘要：

为探讨开花对丹参多倍体丹酚酸积累的影响,以丹参多倍体为材料,探讨花蕾期、盛花期、衰败期叶片中丹酚酸合成关键酶活性的变化。结果表明,可溶性蛋白随着丹参花期的进行,表现出先升后降,在盛花期达到峰值,而5号丹参可溶性蛋白含量在各时期均较其他丹参高。从酪氨酸氨基转移酶(TAT)、肉桂酸-4-羟化酶(C4H)和苯丙氨酸裂解酶(PAL)酶活性变化看,不同丹参在花蕾期TAT酶活性比开花后高,尤其是在盛花期时酶活性明显降低;PAL酶活性基本呈逐渐上升趋势;C4H酶活性变化随着花期的进行先降低后升高;但5号丹参比其他丹参的3种酶活性在各时期高,这可能是其丹酚酸含量较其他丹参高的原因之一。

关键词: 丹参多倍体, 开花, 丹酚酸, 酪氨酸氨基转移酶, 肉桂酸-4-羟化酶, 苯丙氨酸裂解酶

Abstract:

To study the effect of flowering on salvianolic acid accumulation, the changes of the key enzymes activities for salvianolic acid biosynthesis were studied at bud, flowering and decaying stage in Salvia miltiorrhiza polyploid. The results showed that soluble protein increased first and then decreased, and reached the peak at flowering stage of S. miltriorrhiza polyploid, and the soluble protein content in No.5 S. miltriorrhiza leaves was higher than that of other S. miltriorrhiza polyploid at all stages. Tyrosin aminotransferase (TAT) enzyme activity at bud stage was higher than that after flowering, especially low at full flowering stage. The activity of phenylalanine ammonialyase (PAL) enzyme increased gradually in different S. miltriorrhiza polyploid. The change of cinnamic acid 4-hydroxylase (C4H) enzyme activity firstly decreased and then increased during the flowering stage. However, the TAT, PAL and C4H enzyme activities in No.5 S. miltriorrhiza were higher than those of other S. miltriorrhiza polyploid at all stages, which might be one of the reasons why salvianolic acid content in No.5 S. miltriorrhiza was higher than that of others.

Key words: Salvia miltiorrhiza polyploid, flowering, salvianolic acid, tyrosin aminotransferase, cinnamic acid 4-hydroxylase, phenylalanine ammonialyase

中图分类号:

吴顺, 马晓静, 邱云, 孙健鹏, 翟茜, 刘奇. 丹参多倍体开花前后丹酚酸生物合成关键酶活性的变化[J]. 中国农学通报, 2022, 38(19): 73-76.

WU Shun, MA Xiaojing, QIU Yun, SUN Jianpeng, ZHAI Xi, LIU Qi. Changes of Key Enzyme Activities for Salvianolic Acid Biosynthesis Before and After Flowering in Salvia miltiorrhiza Polyploid[J]. Chinese Agricultural Science Bulletin, 2022, 38(19): 73-76.

图/表 4

图1. 丹参开花前后可溶性蛋白含量的变化

图2. 丹参开花前后TAT酶活性的变化

图3. 丹参开花前后PAL活性的变化

图4. 丹参开花前后C4H活性的变化

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丹参多倍体开花前后丹酚酸生物合成关键酶活性的变化

Changes of Key Enzyme Activities for Salvianolic Acid Biosynthesis Before and After Flowering in Salvia miltiorrhiza Polyploid

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