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中国农学通报 ›› 2017, Vol. 33 ›› Issue (10): 43-47.doi: 10.11924/j.issn.1000-6850.casb16050087

• 农学 农业基础科学 • 上一篇    下一篇

10份苎麻种质水培法过冬生长研究

许英,陈建华,栾明宝,王晓飞,孙志民,粟建光   

  1. 中国农业科学院麻类研究所,中国农业科学院麻类研究所,中国农业科学院麻类研究所,中国农业科学院麻类研究所,中国农业科学院麻类研究所,中国农业科学院麻类研究所
  • 收稿日期:2016-05-16 修回日期:2016-07-05 接受日期:2016-07-28 出版日期:2017-04-11 发布日期:2017-04-11
  • 通讯作者: 粟建光
  • 基金资助:
    物种质资源保护(农作物)项目“麻类作物种质资源收集鉴定编目繁种”(2016NWB044);国家麻类产业技术体系“种质资源评价”(CARS- 19-E01);中国农业科学院科技创新工程“麻类作物种质资源与利用”(CAAS-ASTIP-2015-IBFC)。

Growth of Ten Ramie Germplasms in Hydroponics in Winter

  • Received:2016-05-16 Revised:2016-07-05 Accepted:2016-07-28 Online:2017-04-11 Published:2017-04-11

摘要: 为研究低温胁迫下水培法在苎麻上应用可行性,对10 份苎麻种质嫩梢进行水培扦插种植培养,首次研究苎麻水培苗生长在无控温、控湿及加热装置塑料大棚中过冬,其不定根原基、不定根及成活率。结果表明,水培7 天后,10 份苎麻种质均长出不定根原基,有3 份苎麻种质不定根原基生长百分率大于70%,其中‘洞口青皮麻’最高,为100%;不定根与不定根原基呈极显著正相关,相关系数为0.912,有6 份苎麻种质不定根生长百分率大于60.00%,生长最好的为‘宜春铜皮青麻’;过冬后,水培苗成活率与不定根原基、不定根呈显著正相关,相关系数分别为0.845 和0.811;10 份不同基因型水培苗生长分化严重,9 份种质成活、1 份冻死,‘宜春铜皮青麻’、‘洞口青皮麻’及‘马嘴白麻’成活率较高,分别为75.56%、68.89%、60.00%。

关键词: 玉簪, 玉簪, 新品种, 愈伤诱导, 离体快繁, 组织培养

Abstract: In order to obtain the application feasibility of ramie in hydroponics under low temperature stress, in this study, the tender shoots of 10 ramie germplasms are cultivated in hydroponics under plastic greenhouse which had no devices of temperature control, humidity control or heating. We first studied the growth percentage of adventitious root primordia and adventitious roots of ramie germplasm stems and survival rate of hydroponics ramie. The results showed that the stems of 10 ramie germplasms were all long out of adventitious root primordia in hydroponics after 7 days. The growth rates of adventitious root primordia of 3 ramie germplasms were more than 70% , of which‘Dongkou Qing Pi Ma’was 100% . Adventitious roots had extremely and significantly positive correlation with adventitious root primordia. The correlation coefficient was 0.912. There were six ramie germplasms whose adventitious roots growth rates were more than 60.00%, and the best germplasm was‘Yichun Tong Pi Qing Ma’. After winter, hydroponic seedling survival rates of 10 ramie germplasms had significantly positive correlation with adventitious root primordia and adventitious root. The correlation coefficients were 0.845 and 0.811, respectively. Hydroponic seedlings of 10 ramie germplasms grew differently. The survival rates of‘Yichun Tong Pi Qing Ma’,‘Dongkou Qing Pi Ma’and‘Mazui Bai Ma’were better than that of others. The rates were 75.56%, 68.89% and 60.00%, respectively.