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中国农学通报

中国农学通报 ›› 2017, Vol. 33 ›› Issue (34): 63-68.doi: 10.11924/j.issn.1000-6850.casb17090073

所属专题： 生物技术； 园艺

• 生物技术科学 • 上一篇    下一篇

新疆野扁桃自交不亲和基因CULLIN1的克隆及生物信息学分析

王波,余镇藩,曾 斌,夏江宏,马鑫鑫   

  1. 新疆农业大学 林学与园艺学院,新疆农业大学 林学与园艺学院,新疆农业大学 林学与园艺学院,新疆农业大学 林学与园艺学院,新疆农业大学 林学与园艺学院
  • 收稿日期:2017-09-13 修回日期:2017-10-15 接受日期:2017-10-24 出版日期:2017-12-07 发布日期:2017-12-07
  • 通讯作者: 曾 斌
  • 基金资助:
    国家自然科学基金“基于S-核酸酶的新疆野生扁桃自交不亲和性泛素化特性的分子机制研究”(31660557)；新疆维吾尔自治区园艺学重点 学科项目“新疆特色果树种质资源开发利用与优良品种选育推广”(2016-10758-3)。

Self-incompatibility Gene Cullin1 Cloning and Bioinformatics Analysis of Wild Almond in Xinjiang

  • Received:2017-09-13 Revised:2017-10-15 Accepted:2017-10-24 Online:2017-12-07 Published:2017-12-07

摘要： 旨在为今后研究新疆野扁桃自交不亲和性的分子调控奠定良好基础。通过利用Rt-PCR技术及生物测序等方法，从新疆野扁桃的幼嫩叶片中，克隆得到了一个全新的新疆野扁桃Cullin1基因全长cDNA，命名为PetCullin1。并进一步的利用在线软件分析发现：Petcullin1基因全长为2677bp，其开放阅读框2217bp，该基因氨基酸共有738个编码，预测得出Petcullin1蛋白的分子式为C3887H6064N1028O1133S34，原子总数为12096，相对分子质量为85814.61，理论推导出其半衰期为0.8h，带正负电荷的残留的总数分别是113和108，不稳定指数和平均疏水指数分别为40.19、-0.452，该基因蛋白属于水溶性不稳定蛋白。

关键词: 初霜冻日, 初霜冻日, 终霜冻日, 无霜期, 变化特征, 河西走廊东部

Abstract: The aim is to lay a foundation for the future research of molecular regulation of Xinjiang wild almond. A full-length cDNA ofCullin1 gene was cloned from the young leaves of Xinjiang wild almond by using Rt-PCR technique and biological sequencing, and named asPetCullin1 . Further analysis with the online software showed thatPetcullin1 gene was 2677 bp in full-length and its open reading frame was 2217 bp. The amino acid of the gene was predicted to be 738, and the molecular formula of the Petcullin1 protein was predicted to be C3887H6064N1028O1133S34, the total number of atoms was 12096, the relative molecular mass was 85814.61, the theoretical half-time was 30 h. The total number of residues with positive and negative charge was 108 and 113, respectively. The instability index and average hydrophobic index was 40.19 and -0.452 respectively, and the protein belonged to water-soluble labile protein.