生物炭与有机肥施用对黄褐土土壤酶活性及微生物碳氮的影响

doi:10.11924/j.issn.1000-6850.casb18010009

中国农学通报 ›› 2018, Vol. 34 ›› Issue (13): 113-118.doi: 10.11924/j.issn.1000-6850.casb18010009

所属专题：生物技术

• 资源环境生态土壤气象 • 上一篇下一篇

生物炭与有机肥施用对黄褐土土壤酶活性及微生物碳氮的影响

张毅博,韩燕来.,吴名宇,乔丹丹,李慧

河南农业大学资源与环境学院,河南农业大学资源与环境学院;河南省粮食作物协同创新中心,河南农业大学资源与环境学院,河南农业大学资源与环境学院,河南农业大学资源与环境学院

收稿日期:2018-01-02 修回日期:2018-04-11 接受日期:2018-02-24 出版日期:2018-05-07 发布日期:2018-05-07
通讯作者: 李慧
基金资助:
河南省高等学校重点科研项目“黄褐土秸秆还田条件下生物炭减氮增效技术研究”(17A210021)；河南省科技攻关计划项目“保水剂与秸秆配施提高沙质土壤麦玉轮作水氮利用效率关键技术研究”(172102110057)；河南农业大学科研启动经费“秸秆不同处理方式对潮土团聚体特性的影响”(30500593)。

Effect of Biochar and Organic Fertilizer on Soil Enzyme Activities and Microbial Carbon and Nitrogen in Yellow-cinnamon Soil

Received:2018-01-02 Revised:2018-04-11 Accepted:2018-02-24 Online:2018-05-07 Published:2018-05-07

摘要/Abstract

摘要： 研究了生物炭与有机肥配施对土壤酶及其微生物生物量碳氮的影响，为黄褐土障碍因子改良及合理施肥提供参考。在等氮条件下设置了6 个施肥处理：不施肥(CK)、单施生物炭(B)、氮磷钾配合施用(NPK)、氮磷钾+生物炭(NPK+B)、氮磷钾+有机肥(NPK+M)、氮磷钾+有机肥+生物炭(NPK+B+M)。采用氯仿熏蒸-K2SO4浸提法和化学分析法分别对土壤微生物量碳氮、酶活性和土壤化学性质和作物产量进行测定分析。结果表明：在黄褐土条件下，生物炭与有机肥处理均能显著提高土壤脲酶活性和土壤微生物生物量碳、氮含量，其中以NPK+M+B处理效果最好。有机肥能够促进β-葡萄糖苷酶酶活性，而生物炭与其作用相反。土壤微生物量碳氮、脲酶和β-葡萄糖苷酶与产量之间存在显著或极显著正相关关系。本试验条件下，生物炭与有机肥的添加能够提高土壤酶活性，提高土壤有机碳和全氮含量，以NPK+B+M处理对酶活性提升效果最好。

关键词: CMV, CMV, LMoV, RNAi, 植物表达载体, 农杆菌转化

Abstract: The combination effects of biochar and organic fertilizer on soil enzyme activities, microbial biomass carbon and nitrogen were studied to reduce the barriers of roductivity and establish a reasonable fertilization system on yellow-cinnamon soil. Under the condition of equal nitrogen, filed experiments were designed as: no fertilizer (CK), biochar (B), NPK fertilizers (NPK), biochar + NPK fertilizers (NPK+B), organic fertilizer + NPK fertilizers (NPK+M), biochar + organic fertilizer + NPK fertilizers (NPK+B+M). The soil enzyme activities, the microbial biomass carbon (MBC), the microbial biomass nitrogen (MBN) and some properties of soil, yield of wheat were measured by chloroform fumigation- K2SO4 lixiviating method and conventional analysis, respectively. The results showed that both biochar and organic fertilizer increased soil urease activity and enhanced microbial biomass carbon and nitrogen. The treatment NPK+M+B was the best of the all. Organic fertilizer improved β-glucosidase activity while biochar inhibited β-glucosidase activity. The MBC, MBN, soil urease activity and β- glucosidase activity were positively related to the yield significantly. The chemical fertilizer application combined with biochar and organic fertilizer is an optimal way to improve soil enzyme activities in the research.

张毅博,韩燕来.,吴名宇,乔丹丹,李慧. 生物炭与有机肥施用对黄褐土土壤酶活性及微生物碳氮的影响[J]. 中国农学通报, 2018, 34(13): 113-118.

参考文献

[1] 宋长青,吴金水,陆雅海,等. 中国土壤微生物学研究10年回顾[J]. 地球科学进展. 2013(10): 1087-1105.
[2] 胡海波,康立新,梁珍海,等. 泥质海岸防护林土壤酶活性特征研究[J]. 土壤学报. 1998(01): 112-118.
[3] Antal M J, Gr?nli M. The Art, Science, and Technology of Charcoal Production?[J]. Industrial Engineering Chemistry Research. 2003, 42(8): 1619-1640.
[4] Song D, Xi X, Huang S, et al. Short-Term Responses of Soil Respiration and C-Cycle Enzyme Activities to Additions of Biochar and Urea in a Calcareous Soil[J]. PLOS ONE. 2016, 11(9): e161694.
[5] Gul S, Whalen J K. Biochemical cycling of nitrogen and phosphorus in biochar-amended soils[J]. Soil Biology and Biochemistry. 2016, 103: 1-15.
[6] Goldberg E D. Black carbon in the environment: properties and distribution[J]. Environmental Science Technology. 1985.
[7] Marris E. Putting the carbon back: black is the new green[J]. Nature. 2006, 442(7103): 624-626.
[8] El-Naggar A H, Usman A R A, Al-Omran A, et al. Carbon mineralization and nutrient availability in calcareous sandy soils amended with woody waste biochar[J]. Chemosphere. 2015, 138: 67-73.
[9] Zhang A, Liu Y, Pan G, et al. Effect of biochar amendment on maize yield and greenhouse gas emissions from a soil organic carbon poor calcareous loamy soil from Central China Plain[J]. PLANT AND SOIL. 2012, 351(1-2): 263-275.
[10] Keith A, Singh B, Dijkstra F A. Biochar reduces the rhizosphere priming effect on soil organic carbon[J]. Soil Biology and Biochemistry. 2015, 88: 372-379.
[11] Lentz R D, Ippolito J A. Biochar and Manure Affect Calcareous Soil and Corn Silage Nutrient Concentrations and Uptake[J]. JOURNAL OF ENVIRONMENTAL QUALITY. 2012, 41(4): 1033-1043.
[12] 刘领,王艳芳,宋久洋,等. 生物炭与氮肥减量配施对烤烟生长及土壤酶活性的影响[J]. 河南农业科学. 2016(02): 62-66.
[13] Jin Y, Liang X, He M, et al. Manure biochar influence upon soil properties, phosphorus distribution and phosphatase activities: A microcosm incubation study[J]. Chemosphere. 2016, 142: 128-135.
[14] Subedi R, Taupe N, Ikoyi I, et al. Chemically and biologically-mediated fertilizing value of manure-derived biochar[J]. Science of The Total Environment. 2016, 550: 924-933.
[15] 姚丽娟,余有本,周天山,等. 陕南茶园黄褐土的改良研究[J]. 西北农业学报. 2009(05): 316-320.
[16] 关松荫. 土壤酶及其研究法[M]. 中国农业出版社, 1986.
[17] 吴金水. 土壤微生物生物量测定方法及其应用[M]. 气象出版社北京图书发行部, 2006: 150.
[18] 鲍士旦. 土壤农化分析[M]. 中国农业出版社, 2000: 495.
[19] 路文涛,贾志宽,张鹏,等. 秸秆还田对宁南旱作农田土壤活性有机碳及酶活性的影响[J]. 农业环境科学学报. 2011(03): 522-528.
[20] Zhou X, Zhang Y, Downing A. Non-linear response of microbial activity across a gradient of nitrogen addition to a soil from the Gurbantunggut Desert, northwestern China[J]. Soil Biology and Biochemistry. 2012, 47: 67-77.
[21] 赵军,耿增超,尚杰,等. 生物炭及炭基硝酸铵对土壤微生物量碳、氮及酶活性的影响[J]. 生态学报. 2016(08): 2355-2362.
[22] 张阳阳,胡学玉,余忠,等. Cd胁迫下城郊农业土壤微生物活性对生物炭输入的响应[J]. 环境科学研究. 2015(06): 936-942.
[23] 冯爱青,张民,路艳艳,等. 控释氮用量及生物炭对玉米产量及土壤生物化学性质的影响[J]. 水土保持学报. 2014(02): 159-164.
[24] Lehmann J, Rillig M C, Thies J, et al. Biochar effects on soil biota – A review[J]. Soil Biology and Biochemistry. 2011, 43(9): 1812-1836.
[25] 马星竹,陈利军,武志杰,等. 长期施肥土壤β-葡糖苷酶动力学特性研究[J]. 浙江大学学报(农业与生命科学版). 2008(05): 552-556.
[26] Awad Y M, Blagodatskaya E, Ok Y S, et al. Effects of polyacrylamide, biopolymer, and biochar on decomposition of soil organic matter and plant residues as determined by 14C and enzyme activities[J]. European Journal of Soil Biology. 2012, 48: 1-10.
[27] Zhu X, Chen B, Zhu L, et al. Effects and mechanisms of biochar-microbe interactions in soil improvement and pollution remediation: A review[J]. Environmental Pollution. 2017, 227: 98-115.
[28] Yuan J H, Xu R K, Zhang H. The forms of alkalis in the biochar produced from crop residues at different temperatures.[J]. Bioresource Technol. 2011, 102: 3488-3497.
[29] Bailey V L, Fansler S J, Smith J L, et al. Reconciling apparent variability in effects of biochar amendment on soil enzyme activities by assay optimization[J]. Soil Biology and Biochemistry. 2011, 43: 296-301.
[30] Paz-Ferreiro J, Fu S, Méndez A, et al. Biochar modifies the thermodynamic parameters of soil enzyme activity in a tropical soil[J]. Journal of Soils and Sediments. 2015, 15(3): 578-583.
[31] Liu E K, Mei X R, Zhao B Q, et al. Long-term effects of different fertilizer management on microbial biomass C, N and P in a Fluvo-aquic soil.[J]. Journal of China Agricultural University. 2009.
[32] 荣勤雷,梁国庆,周卫,等. 不同有机肥对黄泥田土壤培肥效果及土壤酶活性的影响[J]. 植物营养与肥料学报. 2014(05): 1168-1177.

生物炭与有机肥施用对黄褐土土壤酶活性及微生物碳氮的影响

Effect of Biochar and Organic Fertilizer on Soil Enzyme Activities and Microbial Carbon and Nitrogen in Yellow-cinnamon Soil

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