RNAi 与Bt 毒素协同抗虫技术的应用与展望

doi:10.11924/j.issn.1000-6850.casb18050057

摘要/Abstract

摘要： Bt(Bacillus thuringiensis)毒素优异的抗虫性能和其环境友好性，使其代替了多种化学杀虫剂，在世界范围内得以广泛应用。但同时，过度依赖Bt毒素让许多害虫产生抗性，严重威胁转Bt基因作物和Bt杀虫剂的长期效能；而多种Bt毒素结合应用能产生协同作用或拮抗作用，有不确定性，稳定性较低，无法得到广泛应用。RNAi (RNA interference)技术能沉默特定基因的表达，且特异性强，不会造成环境污染，在害虫防治方面有巨大潜力。Bt毒素和RNAi技术的结合有望在害虫防治方面及抗虫育种的方面开辟一条新道路。为了研究Bt毒素与RNAi技术结合应用的优越性，就Bt毒素和RNAi技术在各自领域的应用及两者相结合应用的最新研究成果等方面进行了综述，总结了近年来RNAi技术与Bt毒素相结合在防控靶标害虫中的应用现状，并对该技术的研究前景提出展望。

关键词: 烤烟, 烤烟, 上部叶, 可用性, 生态条件, 栽培调制, 烘烤工艺

Abstract: Bt (Bacillus thuringiensis) toxin has excellent insect-resistant property and environmental friendliness, which makes Bt toxin a substitution for most chemical pesticides and is widely used worldwide. As a consequence, overusing of Bt toxin has caused pest generating resistant to Bt toxin, and it seriously threatens the long-term effective of Bt crops and Bt insecticides. Although, joint using of various Bt toxins may produce synergistic effect or antagonistic effect, which leads to uncertainty and lower stability. As a result, it can’t be applied widely. RNAi(RNA interference) technology could silence specific gene expression. This technology has strong specificity and it doesn’t cause environmental pollution, which has great potential in pest control. The combined application of Bt toxins and RNAi technology is expected to open a new approach in pest control. In order to study the superiority of joint using of Bt toxin and RNAi technology, this paper reviews the application of Bt toxins and RNAi technology in respective fields, and the latest research of RNAi + Bt application. The states of RNAi + Bt application in controlling target pests is summarized in this paper, and it prospects the future implication of RNAi - Bt technology.

王泳植,王伟重,王锦达. RNAi 与Bt 毒素协同抗虫技术的应用与展望[J]. 中国农学通报, 2018, 34(24): 41-45.

参考文献

[1]Leopoldo Palma,Colin Berry.Understanding the structure and function of Bacillus thuringiensis toxins.Toxicon,2016,109:1-3.
[2]Andrew Fire.RNA-triggered gene silencing.Ternds in Genetics,1999,15(9):358-363.
[3]Mi Ni, Wei Ma, Xiaofang Wang,et al.Next-generation transgenic cotton pyramiding RNAi and Bt counters insect resistance.Plant BiotechnologyJournal,2017,15:1204-1213.
[4]Sarjeet S. Gill, Elizabeth A. Cowles, Patricia V. Pietrantonio.The Mode of Action of Bacillus Thuringiensis Endotoxins,Annual Review of Entomology,1992,37:615-634.
[5]Barbara H. Knowles Julian A. T. Dow.The crystal δ‐endotoxins of Bacillus thuringiensis: Models for their mechanism of action on the insect gut,BioEssays,1993,15(7):469-476.
[6]Sandeep B. GaudanaTamishraha Bagchi.Environmental Impact from the Use of Bt Toxin.Microorganisms in Environmental Management,2011:431-450.
[7]Zenas George,Neil Crickmore.Bacillus thuringiensis Applications in Agriculture.Bacillus thuringiensis Biotechnology,2012:19-39.
[8]Suman Gupta,A. K. Dikshit.Biopesticides: An ecofriendly approach for pest control.Journal of Biopesticides,2010,3(1):186-188.
[9]Laura Massochin Nunes Pinto,Denize Righetto Ziegler,Lidia Mariana Fiuza.The Use of Transgenic Plants in Insect Control. Basic and Applied Aspects of Biopesticides,2014:319-329.
[10]Mahmood-ur-Rahman, Muhammad Qasim, Shazia Anwar Bukhari,et al.Bt Crops: A Sustainable Approach towards Biotic Stress Tolerance.Biological Techniques,2014,1:125-142.
[11]Jie Rang, Hao He, Ting Wang,et al.Comparative Analysis of Genomics and Proteomics in Bacillus thuringiensis 4.0718.PLoS One,2015,10(3):1-15.
[12]Anahi,Alba Monica.RNAi silencing: A tool for functional genomics research on fungi.Fungal Biology Reviews,2016,30(3):91-100.
[13]Daniel H. KimSJohn J. Rossi.RNAi mechanisms and applications.BioTechniques,2008,44(5):613-616.
[14]Ross C.Wilson,Jennifer A.Doudna.Molecular Mechanisms of RNA Interference.Annual Review of Biophysics,2013,42(1):217-239.
[15]MANE, V. A. ,PATIL, A. S.RNAi TO UNDERSTAND AND MANAGE Bt TOXIN RESISTANCE.An International e-Journal,2014,3(2):130-137.
[16]张蕾.植物RNA干扰技术的研究进展及应用前景.农业开发与装备,2017,5:44-44.
[17]Rajan Katoch,Neelam Thakur.RNA interference: a promising technique for the improvement of traditional crops.Food Sciences and Nutrition,2013,64(2):248-259.
[18]Xiaohong Zhu, Gad Galili.Lysine Metabolism Is Concurrently Regulated by Synthesis and Catabolism in Both Reproductive and Vegetative Tissues.Plant Physiology,2004,135:129-136.
[19]Shinjiro Ogita, Hirotaka Uefuji, Yube Yamaguchi.RNA interference: Producing decaffeinated coffee plants.Nature,2003,423:823.
[20]Jiang C J, Shimono M, Maeda S, et al. Suppression of the rice fatty-acid desaturase gene OsSSI2 enhances resistance to blast and leaf blight diseases in rice, Mol Plant Microbe Interact. 2009, 22(7):820-829.
[21]Vijaykumar S. Meli, Sumit Ghosh, T. N. Prabha,et al.Enhancement of fruit shelf life by suppressing N-glycan processing enzymes,Proc Natl Acad Sci U S A,2010,107(6):2413-2418.
[22]Sj?str?m H, Norén O, Olsen J.Structure and function of aminopeptidase N.Advances in Experimental Medicine and Biology.2002,477(2):25-34.
[23]Craig R. Pigott,David J. Ellar.Role of Receptors in Bacillus thuringiensis Crystal Toxin Activity.Microbiology and Molecular Biology Reviews,2007,71:255-281.
[24]Zhi-Zhen Pan,Lian Xu,Bo Liu,et al.PxAPN5 serves as a functional receptor of Cry2Ab in Plutella xylostella (L.) and its binding domain analysis,2017,105(1):516-521.
[25]Wang, X.Y.,SDu, L.X.,SLiu, C.X.,et al.RNAi in the striped stem borer, Chilo suppressalis , establishes a functional role for aminopeptidase N in Cry1Ab intoxication.Journal of invertebrate pathology,2017,143:1-10.
[26]R. Rajagopal,S. Sivakumar,Neema Agrawal,et al.Silencing of Midgut Aminopeptidase N of Spodoptera litura by Double-stranded RNA Establishes Its Role asBacillus thuringiensis Toxin Receptor.J. Biol. Chem.,2002,277(49): 46849-46851.
[27]Yidong Wu.Detection and Mechanisms of Resistance Evolved in Insects to Cry Toxins from Bacillus thuringiensis.Advances in Insect Physiology,2014,47:297-342.
[28]Yang Zhongxia, Wu Qingjun, Wang Shaoli.Silencing of cadherin-like gene in the diamondback moth,Plutella xylostella(Lepidoptera:Plutellidae),using RNAi technique.Acta Entomologica Sinica,2009,52(8):832-837.
[29]周慧丹,杨亦桦,吴益东.RNAi介导的棉铃虫氨肽酶N基因Haapn1和钙粘蛋白基因Ha_BtR沉默对Cry1Ac毒力的影响.昆虫学报,2010,53(10):1097-1103.
[30]Yang Yunlong,Zhu Yu Cheng,Ottea James,et al.Down Regulation of a Gene for Cadherin, but Not Alkaline Phosphatase, Associated with Cry1Ab Resistance in the Sugarcane Borer Diatraea saccharalis.PLoS ONE,2011,6(10):e25783.
[31]Jianghuai Li,Yuemin Ma,Wanli Yuan,et al.FOXA transcriptional factor modulates insect susceptibility to Bacillus thuringiensis Cry1Ac toxin by regulating the expression of toxin-receptor ABCC2 and ABCC3 genes.Insect Biochemistry and Molecular Biology,2017,88:1-11.
[32]Lianet Rodríguez–Cabrera,Damian Trujillo–Bacallao,Orlando Borrás–Hidalgo.RNAi‐mediated knockdown of a Spodoptera frugiperda trypsin‐like serine‐protease gene reduces susceptibility to a Bacillus thuringiensis Cry1Ca1 protoxin.Environmental Microbiology,2010,12(11):2894-2903.
[33]RuobingSGuan,HaichaoSLi,XuexiaSMiao,et al.RNAi pest control and enhanced BT insecticidal efficiency achieved by dsRNA of chymotrypsin-like genes inSOstrinia furnacalis.Journal of Pest Science,2017,90(2):745-757.
[34]Jihyun Hwang, Yonggyun Kim.RNA interference of an antimicrobial peptide, gloverin, of the beet armyworm,Spodoptera exigua, enhances susceptibility to Bacillus thuringiensis.Journal of Invertebrate Pathology,2011,108(3):194-200.
[35]C. Ochoa-Campuzano, M.D. Real, A.C. Martínez-Ramírez,et al.An ADAM metalloprotease is a Cry3Aa Bacillus thuringiensis toxin receptor,Biochem. Biophys. Res. Commun,2007,362:437–442.
[36]Camila Ochoa-Campuzano, Amparo C. Martínez-Ramírez, Estefanía Contreras.Prohibitin, an essential protein for Colorado potato beetle larval viability,is relevant to Bacillus thuringiensis Cry3Aa toxicity.Pesticide Biochemistry and Physiology.2013,107:299-308.
[37]Hyun Soo Kim,Soyoung Noh,Youngjin Park.Enhancement of Bacillus thuringiensis Cry1Ac and Cry1Ca toxicity against Spodoptera exigua (Hübner) by suppression of a chitin synthase B gene in midgut.Journal of Asia-Pacific Entomology,2017,20(1):199-205.
[38]H. Keown,M. O′Callaghan,L.G. Greenfield.Decomposition of nucleic acids in soil.New Zealand Natural Sciences,2004,29:13-19.
[39]Saxena S,Jónsson Z O,Dutta A.Small RNAs with imperfect match to endogenous mRNA repress translation implications for off-target activity of small inhibitory RNA in mammalian cells.Journal of Biological Chemistry,2003,278(45):44312-44319.
[40]Kang-lai HE,Zhen-ying WANG,Ping SHEN.Progress for environmental risk assessments of RNAi-based crops.Journal of Biosafety,2014,23(4):238-247.
[41]焦悦,付伟,翟勇.RNAi技术在作物中的应用及安全评价研究.作物杂志,2018(1):9-15.
[42]R. Miethling-Graff,S. Dockhorn,C.C. Tebbe.Release of the recombinant Cry3Bb1 protein of Bt maize MON88017 into field soil and detection of effects on the diversity of rhizosphere bacteria, Eur. J. Soil Biol. 2010,46:41–48.
[43]Y. Li,K. Wu,Y. Zhang,et al.Degradation of Cry1Ac protein within transgenic Bacillus thuringiensis rice tissues under field and laboratory conditions.Environ. Entomol,2007,36:1275–1282.
[44]M.J. Zhang, M.C. Feng, L.J. Xiao,et al.Impact of water content and temperature on the degradation of Cry1Ac protein in leaves and buds of Bt cotton in the soil.PLoS One 10,2015, e115240.
[45]Carmen Sara Hernández Rodríguez,Aaron Cabrera Asensio,Primitivo Caballero,et al.Encapsulation of the Bacillus thuringiensis secretable toxins Vip3 Aa and Cry1 Ia in Pseudomonas fluorescens.Biol Control, 2013,66:65-159.
[46]Leopoldo Palma.Bacillus thuringiensis-based biopesticides, are they as effective as they should be?.Rev Argent Microbiol,2017,49(1):119-120.