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中国农学通报

中国农学通报 ›› 2019, Vol. 35 ›› Issue (2): 62-67.doi: 10.11924/j.issn.1000-6850.casb18080030

• 林学 园艺 园林 • 上一篇    下一篇

小滨菊组培快繁体系的初步建立

邵冰洁1, 刘文竹1, 许建新2, 高永慧3, 赵惠恩1   

  1. 1.北京林业大学;2.深圳市铁汉生态环境有限公司;3.辽宁省兴城市农业广播电视学校
  • 收稿日期:2018-08-08 修回日期:2018-12-24 接受日期:2018-09-25 出版日期:2019-01-11 发布日期:2019-01-11
  • 通讯作者: 赵惠恩
  • 基金资助:
    北京市科技计划课题“延庆地区药食同源菊花、玫瑰品种培育和产业化关键技术的研发与应用”(Z181100002518003)。

Preliminary Establishment of Rapid Propagation of Leucanthemella linearis by Tissue Culture

  • Received:2018-08-08 Revised:2018-12-24 Accepted:2018-09-25 Online:2019-01-11 Published:2019-01-11

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摘要： 以多年生沼生植物小滨菊（Leucanthemella linearis (Matsum.) Tzvel.）的带芽茎段为外植体，开展了小滨菊组培快繁体系建立的研究。结果表明，小滨菊茎段的最佳启动培养基为MS+0.5 mg?L-1 6-BA + 0.5 mg?L-1 NAA，启动率达到80%；最佳继代培养基为MS+0.5 mg?L-1 6-BA+0.2 mg?L-1 NAA，增殖效果良好，出芽指数平均达到9.2；最佳生根培养基为1/2MS+0.3 mg?L-1 NAA，生根率85.72%，根系生长状态良好。小滨菊茎段诱导愈伤组织的最佳培养基为MS+0.2 mg?L-1 6-BA+0.5 mg?L-1 NAA，愈伤组织分化率最高的培养基为MS+1.0 mg?L-1 6-BA+0.2 mg?L-1 NAA，分化率可达70.00%。

关键词: 极端降水事件, 极端降水事件, 时空分布, 农业生产, 结构向量自回归, 动态影响

Abstract: Experiments were conducted for the standardization of micropropagation and regeneration of Leucanthemella linearis, a helophyte perennial plant. Nodal segment were used as explants and they were cultured on MS medium and 1/2MS medium supplemented with different concentrations of 6-BA and NAA both in individual and in combined form for shoot inductions and the best result of primary cultural was obtained from MS medium supplemented with 6-BA and NAA at the concentrations of 0.5 mg?L-1 and 0.5 mg?L-1 respectively. The best result of subcultural was obtained from MS medium supplemented with 6-BA+NAA at the concentrations of 0.5 mg?L-1 and 0.2 mg?L-1 respectively, resulting about 9.2 multiple shoots from a single shoot tip explant. For root induction and elongation, 1/2MS medium plus NAA at the concentrations of 0.3 mg?L-1showed an adverse effect. Callus was induced from nodal segment on MS medium supplemented with 0.2 mg?L-16-BA and 0.5 mg?L-1 NAA, and plants regenerated best from these calluses on MS medium supplemented with 1.0 mg?L-1 6-BA and 0.2 mg?L-1 NAA, the ratio of regeneration was 70.00%.