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中国农学通报

中国农学通报 ›› 2019, Vol. 35 ›› Issue (7): 134-138.doi: 10.11924/j.issn.1000-6850.casb18100041

所属专题： 园艺

• 食品 营养 检测 安全 • 上一篇    下一篇

亲水性液相色谱串联质谱法测定红甜菜中甜菜碱含量

周芹, 吴玉梅   

  1. 黑龙江大学农作物研究院
  • 收稿日期:2018-10-12 修回日期:2018-11-01 接受日期:2018-11-09 出版日期:2019-03-04 发布日期:2019-03-04
  • 通讯作者: 吴玉梅
  • 基金资助:
    国家甜菜现代农业产业技术体系项目“质量安全与营养品质评价”(CARS-170503)；国家农产品质量安全风险评估专项经费“糖料食用特 色农产品质量安全风险因子摸底排查与特质性营养品质评价”(GJFP201801002)。

Determination of betaine in red Sugarbeet by hydrophilic interaction liquid chromatography-tandem mass spectrometryZhou Qin<sup>1,2,3,4</sup>, Wu Yumei<sup>1,2,3,4</sup>

  • Received:2018-10-12 Revised:2018-11-01 Accepted:2018-11-09 Online:2019-03-04 Published:2019-03-04

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摘要： 为了建立红甜菜中甜菜碱的定量分析方法，采用改进的QuEChERS方法结合液相色谱串联质谱技术，样品经快速溶剂萃取仪以甲醇为溶剂提取，经固相萃取柱净化，采用正向亲水性液相色谱柱(Hilic)，梯度洗脱，通过质谱的多反应监测(multiple reactions monitoring，MRM)正离子模式扫描进行测定，外标法定量。结果表明：甜菜碱在本方法的线性范围为0.05~5 ug/mL，添加水平为4~16 mg/g时，回收率为87.7%~95.7%，方法RSD值为3.69%~8.4%，检出限为0.01 mg/L，定量限为0.04 mg/L，与传统的分析方法相比，具有检出限低、灵敏度高的优点，完全可以适用于甜菜中甜菜碱的分析。

关键词: 气温日变化, 气温日变化, 变温, 城郊差异

Abstract: The study aims to establish a quantitative analysis method for detecting betaine in red sugarbeet. By using the modified QuEChERS method combined with liquid chromatography-tandem mass spectrometry, the sample was extracted with methanol through accelerated solvent extraction(ASE), and purified by solid phase extraction, detected by hydrophilic interaction liquid chromatography- tandem mass spectrometry under multiple reaction monitoring mode and quantified by the external standard method. The results showed that linearity range was 0.05-5 μg/mL in the test. When the adding level was 4-16 mg/g, the recovery was 87.7%- 95.7%, and RSD was between 3.6% and 8.4%. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.01 and 0.04 mg/L, respectively. The method is easy, quick, highly sensitive, and suitable for determination of betaine in sugabeet.

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