外源谷胱甘肽对低温胁迫下玉米幼苗的影响

doi:10.11924/j.issn.1000-6850.casb2020-0764

摘要/Abstract

摘要：

试验旨在研究外源谷胱甘肽增强玉米幼苗防御低温胁迫的效应。选取生产上大面积推广的‘郑单958’品种为试验材料,采用土培法,设置6组处理,分别为CK、LT、G1、G2、G3、G4,每个处理3次重复。将培养至三叶一心的玉米幼苗分别进行叶面喷施3天后,在人工气候箱中采用10℃低温胁迫,并对玉米幼苗生长及相关生理指标进行分析。结果表明,喷施外源谷胱甘肽能够显著减轻低温胁迫对玉米幼苗苗高、叶片含水量及叶绿素含量的影响。低温胁迫5天后,谷胱甘肽通过增强过氧化物酶活性缓解低温胁迫对玉米幼苗的伤害,G3与LT处理存在明显差异,提高了79.25%;在低温天数的增加下,玉米叶片细胞内可溶性糖及丙二醛含量逐渐升高,胁迫7天后,LT处理与胁迫后第5天相比,可溶性糖及丙二醛含量分别增加了106.97%、25.33%。综合来看,外源谷胱甘肽能提高低温胁迫下玉米的生长能力,主要通过提高抗氧化酶和光合调控能力,保持细胞渗透平衡。

关键词: 玉米, 低温胁迫, 外源谷胱甘肽, 幼苗生长, 生理特性

Abstract:

The purpose of this experiment is to study the effect of exogenous glutathione on enhancing the defense of corn seedlings against low temperature stress. The maize variety ‘Zhengdan 958’ was used as material in this study. Soil culture method was carried out with six treatments (CK, LT, G1, G2, G3 and G4), and each treatment was repeated three times. The maize seedlings with three leaves had leaf-spray for 3 days, and then transferred to artificial climate box with 10℃ low temperature stress. The growth traits of maize seedlings and the related physiological indexes were analyzed. The results show that spraying exogenous glutathione could significantly reduce the effects of low temperature stress on maize seedling height, leaf water content and chlorophyll content. Glutathione could alleviate the oxidation damage of maize seedlings under low temperature stress through enhancing the activity of antioxidant enzymes. There is ignificant difference between LT and G3 whose antioxidant enzyme activities are increased by 79.25%. With the increase of low temperature stress days, the contents of soluble sugar and malondialdehyde in maize leaves increase gradually. Compared with the fifth day after stress, the contents of soluble sugar and malondialdehyde on the seventh day are increased by 106.97% and 25.33%, respectively. Taken together, exogenous glutathione can improve the growth ability of maize under low temperature stress, mainly by increasing the activity of antioxidant enzymes and photosynthetic regulation ability, and maintaining cell osmotic balance.

Key words: maize, low temperature stress, exogenous glutathione, seedling growth, physiological characteristics

中图分类号:

S513

李林, 杜卓, 侯雯, 张凯, 黄浩, 路运才. 外源谷胱甘肽对低温胁迫下玉米幼苗的影响[J]. 中国农学通报, 2021, 37(27): 16-20.

Li Lin, Du Zhuo, Hou Wen, Zhang Kai, Huang Hao, Lu Yuncai. Exogenous Glutathione: Effects on Maize Seedlings Under Low Temperature Stress[J]. Chinese Agricultural Science Bulletin, 2021, 37(27): 16-20.

图/表 6

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处理条件	苗高/cm
处理条件	0 d	3 d	5 d	7 d
CK	35.83±0.40a	43.23±0.21a	45.13±0.25a	47.77±0.25a
LT	35.97±0.61a	37.23±0.25d	37.60±0.36d	37.83±0.42d
G1	35.80±0.10a	40.53±0.25b	40.77±0.25b	41.00±0.27b
G2	36.17±0.25a	39.40±1.23c	40.50±0.87bc	40.50±0.87bc
G3	35.45±0.44a	38.57±0.21e	39.07±0.38e	39.30±0.61e
G4	35.73±0.15a	39.63±0.32bc	39.73±0.32c	39.83±0.31c

处理条件	苗高/cm
处理条件	0 d	3 d	5 d	7 d
CK	35.83±0.40a	43.23±0.21a	45.13±0.25a	47.77±0.25a
LT	35.97±0.61a	37.23±0.25d	37.60±0.36d	37.83±0.42d
G1	35.80±0.10a	40.53±0.25b	40.77±0.25b	41.00±0.27b
G2	36.17±0.25a	39.40±1.23c	40.50±0.87bc	40.50±0.87bc
G3	35.45±0.44a	38.57±0.21e	39.07±0.38e	39.30±0.61e
G4	35.73±0.15a	39.63±0.32bc	39.73±0.32c	39.83±0.31c

处理条件	叶片含水量/%
处理条件	0 d	3 d	5 d	7 d
CK	90.40±0.03a	85.63±0.29a	82.68±0.15a	81.40±0.18ab
LT	90.13±0.16a	84.85±0.38a	81.69±3.63a	80.48±2.14bc
G1	90.17±0.03a	85.54±0.47a	82.98±0.67a	82.23±2.38ab
G2	90.08±0.08a	85.89±0.77a	82.93±1.37a	81.55±0.46ab
G3	90.10±0.01a	86.52±0.63a	84.25±0.38a	83.47±0.28a
G4	90.08±0.08a	85.67±2.56a	82.30±3.05a	83.00±1.55a

处理条件	叶片含水量/%
处理条件	0 d	3 d	5 d	7 d
CK	90.40±0.03a	85.63±0.29a	82.68±0.15a	81.40±0.18ab
LT	90.13±0.16a	84.85±0.38a	81.69±3.63a	80.48±2.14bc
G1	90.17±0.03a	85.54±0.47a	82.98±0.67a	82.23±2.38ab
G2	90.08±0.08a	85.89±0.77a	82.93±1.37a	81.55±0.46ab
G3	90.10±0.01a	86.52±0.63a	84.25±0.38a	83.47±0.28a
G4	90.08±0.08a	85.67±2.56a	82.30±3.05a	83.00±1.55a