丛枝菌根真菌(AMF)接种剂种类和接种时期对牡丹菌根化组培苗培养的影响

doi:10.11924/j.issn.1000-6850.casb2021-0628

摘要/Abstract

摘要：

为探索丛枝菌根真菌(AMF)在牡丹组培中的应用,解决牡丹组培苗生根难、移栽成活率低等瓶颈问题,培育优质牡丹菌根化组培苗,以多个牡丹品种和杂交系组培苗为接种对象,设计筛选AMF接种剂种类和接种时期的最佳组合。结果表明：瓶内接种,在根长≥2.0 cm时接种AMF的菌根苗,AM菌根的侵染率与侵染强度最高,根长和根数分别比不接种AMF（对照）增长了49.29%和46.99%。AMF对不同品种的影响不一致,对于‘Z1-10-3’,接种混合孢子Glomus geosporum+Glomus mosseae(Gg+Gm)的侵染率(93.12%)与侵染强度(31.23)最高,极显著(P=0.01)高于单一接种剂Glomus mosseae(Gm)和Glomus geosporum(Gg);‘Z1-WM’接种Gg+Gm的侵染率(95.96%)与侵染强度(33.33)也最高,其次是Gg,然后是Gm;对于‘乌龙捧盛’,接种Gg的侵染率(85.76%)和侵染强度(25.80)最高。瓶内接种AMF能更有效地提高牡丹组培苗移栽成活率45.88个百分点。因此,牡丹组培苗在瓶内根长≥2.0 cm时接种混合AMF孢子,可实现组培苗菌根化过程,且其移栽成活率有效提高。

关键词: 牡丹, 丛枝菌根真菌, 组培苗, 菌根化, 侵染, 移栽成活率

Abstract:

To explore the arbuscular mycorrhizal fungi (AMF) in the application of Paeonia suffruticosa tissue culture and solve the bottleneck problems such as difficult rooting of in vitro plants and low transplantation survival rate etc, we cultivated high-quality mycorrhizal plantlets of P. suffruticosa based on different P. suffruticosa cultivars and hybrids, and selected the best combination of dominant species of AMF, inoculation type and inoculation period. The results showed that: the frequency and intensity of colonization of mycorrhiza inoculated with AMF were the highest among all the treatments after the root length was ≥2.0 cm in vitro. The length and number of mycorrhiza were 49.29% and 46.99% higher than that of the control (without inocultating AMF). As for ‘Z1-10-3’, inocultating the mixture spore of Glomus geosporum+Glomus mosseae (Gg+Gm) had the highest frequency (93.12%) and intensity of colonization (31.23), extremely and significantly higher than that of Gg and Gm (P=0.01); and for ‘Z1-WM’, the mixture spore of (Gg+Gm) had the highest frequency (95.96%) and intensity of colonization (33.33), followed by Gg, then Gm; for ‘Wulongpengsheng’, inocultating Gg had the highest frequency (85.76%) and intensity of colonization (25.80). The inoculation of AMF in vitro could increase the survival rate of transplantation for vitroplants by 45.88 percentage points compared with that of the control. Therefore, with the inoculation of AMF, the mycorrhization of vitroplants of P. suffruticosa could be realized, it could increase the transplanting survival rate and promote the growth of mycorrhizal tissue culture plantlets of P. suffruticosa effectively.

Key words: Paeonia suffruticosa, arbuscular mycorrhizal fungi, tissue culture plantlet, mycorrhizal, colonization, transplanting survival rate

中图分类号:

S685.11

曾端香, 袁涛, 王莲英. 丛枝菌根真菌(AMF)接种剂种类和接种时期对牡丹菌根化组培苗培养的影响[J]. 中国农学通报, 2021, 37(30): 53-58.

Zeng Duanxiang, Yuan Tao, Wang Lianying. Effects of Arbuscular Mycorrhizal Fungi (AMF) Species and Inoculating Period on Mycorrhizal Tissue Culture Plantlets of Paeonia suffruticosa[J]. Chinese Agricultural Science Bulletin, 2021, 37(30): 53-58.

图/表 5

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分级	侵染比例
0级	0%
1级	0%<n≤1%
2级	1%<n≤10%
3级	10%<n≤50%
4级	50%<n≤90%
5级	>90%

分级	侵染比例
0级	0%
1级	0%<n≤1%
2级	1%<n≤10%
3级	10%<n≤50%
4级	50%<n≤90%
5级	>90%

编号	时期	处理	侵染率/%	侵染强度	生根率/%	平均根长/cm	平均根数
休眠芽组培苗Ⅰ	未诱导生根阶段	对照	0.00Cc	0.00Cd	10.20Cd	0.21Dd	0.52Dd
休眠芽组培苗Ⅰ	未诱导生根阶段	AMF	2.11Cc	1.32Ccd	16.80Cd	0.24Dd	0.63Dd
休眠芽组培苗Ⅱ	已完成根诱导阶段	对照	0.00Cc	0.00Ccd	55.23Bc	2.25Cc	1.72Dd
休眠芽组培苗Ⅱ	已完成根诱导阶段	AMF	58.00Bb	16.45Bb	73.50Bb	2.85BCb	2.17Bb
休眠芽组培苗Ⅲ	根长2 cm以上	对照	0.00Cc	0.00Cc	100.00Aa	2.82Bb	1.83Cc
休眠芽组培苗Ⅲ	根长2 cm以上	AMF	78.25Aa	23.33Aa	100Aa	4.21Aa	2.69Aa

编号	时期	处理	侵染率/%	侵染强度	生根率/%	平均根长/cm	平均根数
休眠芽组培苗Ⅰ	未诱导生根阶段	对照	0.00Cc	0.00Cd	10.20Cd	0.21Dd	0.52Dd
休眠芽组培苗Ⅰ	未诱导生根阶段	AMF	2.11Cc	1.32Ccd	16.80Cd	0.24Dd	0.63Dd
休眠芽组培苗Ⅱ	已完成根诱导阶段	对照	0.00Cc	0.00Ccd	55.23Bc	2.25Cc	1.72Dd
休眠芽组培苗Ⅱ	已完成根诱导阶段	AMF	58.00Bb	16.45Bb	73.50Bb	2.85BCb	2.17Bb
休眠芽组培苗Ⅲ	根长2 cm以上	对照	0.00Cc	0.00Cc	100.00Aa	2.82Bb	1.83Cc
休眠芽组培苗Ⅲ	根长2 cm以上	AMF	78.25Aa	23.33Aa	100Aa	4.21Aa	2.69Aa

编号	根长	处理	侵染率/%	侵染强度
胚根苗Ⅰ	根长≤0.5 cm	对照	0.00Dd	0.00Ee
胚根苗Ⅰ	根长≤0.5 cm	AMF	35.23Cc	9.21Cc
胚根苗Ⅱ	0.5 cm≤根长≤1.0 cm	对照	0.00Dd	0.00Dd
胚根苗Ⅱ	0.5 cm≤根长≤1.0 cm	AMF	67.20Bb	18.05Bb
胚根苗Ⅲ	根长≥2.0 cm	对照	0.00Dd	0.00Dd
胚根苗Ⅲ	根长≥2.0 cm	AMF	90.25Aa	30.33Aa