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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (26): 8-16.doi: 10.11924/j.issn.1000-6850.casb2022-0834

• 农学·农业基础科学 • 上一篇    下一篇

大豆种质资源萌发期耐盐性评价和耐盐机理解析

王萌1(), 刘文君2, 鲁雪莉3,4, 陈庆山5, 杨明亮5, 吕波6, 徐宗昌3,4()   

  1. 1 青岛农业大学农学院，山东青岛 266109
    2 河北科技师范学院海洋资源与环境学院，河北秦皇岛 066044
    3 中国农业科学院烟草研究所海洋农业研究中心，山东青岛 266100
    4 青岛市滨海盐碱地资源挖掘与生物育种重点实验室，山东青岛 266100
    5 东北农业大学农学院，哈尔滨 150000
    6 山东农业大学生命科学学院，山东泰安 271018
  • 收稿日期:2022-10-09 修回日期:2023-01-15 出版日期:2023-09-15 发布日期:2023-09-11
  • 通讯作者: 徐宗昌，男，1988年出生，山东即墨人，助理研究员，博士，主要从事大豆遗传育种研究。通信地址：266100 山东省青岛市崂山区科苑经四路11号 中国农业科学院烟草研究所海洋农业研究中心，E-mail：xuzongchang@caas.cn。
  • 作者简介:

    王萌，女，1989年出生，山东郓城人，讲师，博士，主要从事大豆种质资源耐盐鉴定研究。通信地址：266109 山东省青岛市城阳区长城路700号 青岛农业大学农学院，E-mail：。

  • 基金资助:
    中国农业科学院科技创新工程“盐碱地产能提升关键技术与集成示范”(CAAS-ZDRW202201); 青岛市科技惠民示范引导专项“基于耐盐植物和土壤调理剂的滨海盐渍土改良模式与应用”(20-3-4-7-nsh)

Soybean Germplasm Resources at Germination: Salt Tolerance Evaluation and Mechanism Analysis

WANG Meng1(), LIU Wenjun2, LU Xueli3,4, CHEN Qingshan5, YANG Mingliang5, LV Bo6, XU Zongchang3,4()   

  1. 1 College of Agriculture, Qingdao Agricultural University, Qingdao, Shandong 266109
    2 College of Marine Resources & Environment, Hebei Normal University of Science & Technology, Qinhuangdao, Hebei 066044
    3 Marine Agriculture Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, Shandong 266100
    4 Qingdao Key Laboratory of Resources Mining and Biological Breeding in Coastal Saline-alkali Land, Qingdao, Shandong 266100
    5 College of Agriculture, Northeast Agricultural University, Harbin 150000
    6 College of life sciences, Shandong Agricultural University, Taian, Shandong 271018
  • Received:2022-10-09 Revised:2023-01-15 Published-:2023-09-15 Online:2023-09-11

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摘要：

为了筛选耐盐大豆种质资源作为耐盐育种亲本材料和盐碱地种植的依据，用0.4%和0.8%的NaCl处理52份大豆种质，调查萌发指标和部分种质的苗期指标，进行耐盐评价。结果表明，0.8% NaCl处理下，群体发芽势、发芽率、下胚轴长和粗等指标较对照和0.4% NaCl处理显著下降，但群体内个体差异较大。在0.4%和0.8% NaCl胁迫条件下，不耐盐大豆种质的叶绿素含量和叶片相对含水量均显著低于耐盐种质；在0.8% NaCl胁迫下，不耐盐种质根中持Na+能力降低，茎中的Na+含量显著高于耐盐种质。通过隶属函数法综合分析，共筛选出耐盐大豆种质资源18份，中间型种质资源23份，不耐盐种质资源11份。

关键词: 大豆, 耐盐性, 种质资源, 萌发期, 隶属函数法

Abstract:

In order to select salt-tolerant soybean germplasm as the foundation for salt-tolerance breeding, 52 soybean genotypes were exposed to 0.4% of NaCl and 0.8% of NaCl, and the germination indices and some seedling stage indices were investigated for salt tolerance. The results showed that the germination potential, germination rate, hypocotyl length and diameter of the population were decreased significantly under 0.8% of NaCl treatment, compared with the control and 0.4% of NaCl treatment, but there were significant differences among individuals in the population. Salt-intolerant soybean germplasm had significantly lower chlorophyll content and leaf relative water content under 0.4% and 0.8% of NaCl stress than salt-tolerant soybean germplasm. The ability of holding Na+ in the roots in salt-intolerant germplasm was decreased under 0.8% of NaCl stress, while Na+ contents in the stems were higher than that of salt-tolerant germplasm. A total of 18 salt-tolerant soybean germplasm materials, 23 intermediate soybean germplasm materials and 11 salt-intolerant soybean germplasm materials were screened by membership function method.

Key words: soybean, salt resistance, germplasm resources, germination period, membership function method