不同波长LEDs光源对苦荞芽菜生长、生物活性物积累及抗氧化活性的影响

doi:10.11924/j.issn.1000-6850.casb2023-0600

中国农学通报 ›› 2024, Vol. 40 ›› Issue (12): 45-52.doi: 10.11924/j.issn.1000-6850.casb2023-0600

所属专题：园艺

不同波长LEDs光源对苦荞芽菜生长、生物活性物积累及抗氧化活性的影响

陈丽丽¹^,²^,³^,⁴(), 吕平², 吕少杰², 房明志², 李斌², 胡跃高³, 薛绪掌⁵, 康文艺¹()

¹ 河南大学农学院，河南开封 475000
² 浙江道济农业科技发展有限公司，浙江嘉兴 314504
³ 中国农业大学农学院，北京 100193
⁴ 宁波大学科学技术学院，浙江宁波 315300
⁵ 北京市农林科学院，农业智能装备研究中心，北京 100097

收稿日期:2023-08-22 修回日期:2023-11-16 出版日期:2024-04-25 发布日期:2024-04-22
通讯作者:
康文艺，男，二级教授，博导，博士，主要从事药用、食用植物化学成分及活性研究。通信地址：475001 河南省开封市河南大学金明校区，Tel：0371-23832100，E-mail：kangweny@hotmail.com。
作者简介:
陈丽丽，女，1988年出生，河南兰考人，博士，主要从事人工光源调控植物生长发育及次生代谢物合成的研究。通信地址：315300 浙江省宁波市慈溪市白沙路街道文蔚路521号，Tel：19857455253，E-mail：chenlili2@nbu.edu.cn。
基金资助:
国家农业产业技术体系燕荞麦东北栽培岗“燕荞麦优质高效栽培”(2016AA103005); 宁波大学科学技术学院高层次人才科研启动经费“光源调控植物生长发育”(RC100009)

Effects on Growth, Bioactive Substance Accumulation and Antioxidant Activity of Tartary Buckwheat Sprouts: Different Wavelengths of LEDs Light

CHEN Lili¹^,²^,³^,⁴(), LV Ping², LV Shaojie², FANG Mingzhi², LI Bin², HU Yuegao³, XUE Xuzhang⁵, KANG Wenyi¹()

¹ School of Agriculture, Henan University, Kaifeng, Henan 475000
² Zhejiang Daoji Agricultural Science and Technology Development Co., LTD, Jiaxing, Zhejiang 314504
³ College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193
⁴ College of Science &Technology, Ningbo University, Ningbo, Zhejiang 315300
⁵ Beijing Research Center for Intelligent Equipment in Agriculture, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097

Received:2023-08-22 Revised:2023-11-16 Published:2024-04-25 Online:2024-04-22

摘要/Abstract

摘要：

为探究不同波长LEDs光源对苦荞芽菜生长的影响，本研究以黑暗(D)作对照，采用单色红(R)、蓝(B)、绿(G)光源培养苦荞芽菜，收获后分别测定其形态指标、生物活性物积累量、类黄酮合成关键基因表达量及抗氧化活性指标，结果表明：B处理的苦荞芽菜下胚轴呈深红色，伸长生长受到强烈抑制，花青素、总黄酮及总酚积累量均最高，分别是D的7.18、2.96和2.49倍；R处理的下胚轴呈浅粉色，上述3种生物活性物积累量分别是对照的2.56、1.68和1.40倍；G处理的下胚轴伸长与R相似，外观呈透明白色，生物活性物积累量与对照无显著差异。B和R均强烈诱导类黄酮生物合成通路上关键结构基因表达，显著增加苦荞芽菜下胚轴抗氧化活性。此外，各单色光处理均未显著影响苦荞芽菜可食用部位的鲜重。由此可见，红、蓝LEDs光源可显著影响苦荞芽菜形态生长，并增加其生物活性物积累及抗氧化能力，以蓝光最佳。

关键词: 红/蓝/绿LEDs光源, 苦荞芽菜, 生物活性物, 抗氧化活性, 类黄酮合成基因表达

Abstract:

In order to investigate the effects of different wavelengths of LEDs light sources on the growth of tartary buckwheat sprouts, red (R), blue (B) and green (G) LEDs were used to culture the sprouts, with the darkness as a control. The indicators including morphological growth, bioactive matter accumulation, key gene expression involved in flavonoid synthesis, and antioxidant activity were determined, respectively. The results showed that the hypocotyls of tartary buckwheat sprouts under B were deep red and the hypocotyl elongation was strongly inhibited. The accumulation of anthocyanins, total flavonoids and total phenols under B were the highest, which were 7.18, 2.96 and 2.49 times as that of D, respectively. The hypocotyl of tartary buckwheat sprouts under R showed a light pink color. The accumulation of the above three bioactive substances under R was also significantly higher than that of the control, which was 2.56, 1.68 and 1.40 times, respectively. The hypocotyl elongation of the sprouts under G was similar to that of R, but the appearance was transparent and white. The accumulation of bioactive matters under G was not significantly different from that of the control. Both B and R strongly induced the expression of key genes involved in flavonoid biosynthesis pathway, and significantly increased the antioxidant activity of the hypocotyls of tartary buckwheat sprouts. In conclusion, red and blue LEDs could significantly affect the morphological growth of tartary buckwheat sprouts, and increase the accumulation of bioactive substances as well as the antioxidant capacity. Meanwhile B treatment was recommended due to the best performance.

Key words: red/blue/green LEDs light source, tartary buckwheat sprouts, bioactive substance, antioxidant activity, flavonoid synthesis gene expression

陈丽丽, 吕平, 吕少杰, 房明志, 李斌, 胡跃高, 薛绪掌, 康文艺. 不同波长LEDs光源对苦荞芽菜生长、生物活性物积累及抗氧化活性的影响[J]. 中国农学通报, 2024, 40(12): 45-52.

CHEN Lili, LV Ping, LV Shaojie, FANG Mingzhi, LI Bin, HU Yuegao, XUE Xuzhang, KANG Wenyi. Effects on Growth, Bioactive Substance Accumulation and Antioxidant Activity of Tartary Buckwheat Sprouts: Different Wavelengths of LEDs Light[J]. Chinese Agricultural Science Bulletin, 2024, 40(12): 45-52.

图/表 6

参考文献 32

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指标	处理
指标	D	G	R	B
子叶鲜重/mg	190.26±12.13ab	182.18±12.57b	205.48±13.98a	214.35±13.27a
子叶干重/mg	35.31±7.55a	27.91±8.46b	26.45±8.37b	31.26±8.22ab
下胚轴长/cm	10.93±1.01a	9.25±0.89ab	9.33±0.91ab	6.62±0.39c
下胚轴直径/mm	1.08±0.01a	1.02±0.01a	1.12±0.01a	1.01±0.01a
下胚轴鲜重/mg	771.72±13.24a	740.54±16.78a	777.18±17.23a	693.61±6.44b
下胚轴干重/mg	32.39±2.23a	31.52±3.29ab	31.87±2.52ab	31.38±1.93ab
食用部分鲜重/mg	960.35±12.49a	922.36±14.01ab	985.38±19.24a	922.99±14.33ab
食用部分干重/mg	68.23±4.18a	59.78±3.33b	58.25±4.28b	63.54±3.16a

指标	处理
指标	D	G	R	B
子叶鲜重/mg	190.26±12.13ab	182.18±12.57b	205.48±13.98a	214.35±13.27a
子叶干重/mg	35.31±7.55a	27.91±8.46b	26.45±8.37b	31.26±8.22ab
下胚轴长/cm	10.93±1.01a	9.25±0.89ab	9.33±0.91ab	6.62±0.39c
下胚轴直径/mm	1.08±0.01a	1.02±0.01a	1.12±0.01a	1.01±0.01a
下胚轴鲜重/mg	771.72±13.24a	740.54±16.78a	777.18±17.23a	693.61±6.44b
下胚轴干重/mg	32.39±2.23a	31.52±3.29ab	31.87±2.52ab	31.38±1.93ab
食用部分鲜重/mg	960.35±12.49a	922.36±14.01ab	985.38±19.24a	922.99±14.33ab
食用部分干重/mg	68.23±4.18a	59.78±3.33b	58.25±4.28b	63.54±3.16a

不同波长LEDs光源对苦荞芽菜生长、生物活性物积累及抗氧化活性的影响

Effects on Growth, Bioactive Substance Accumulation and Antioxidant Activity of Tartary Buckwheat Sprouts: Different Wavelengths of LEDs Light

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