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中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (25): 52-58.doi: 10.11924/j.issn.1000-6850.casb2023-0832

• 林学·园艺·园林 • 上一篇    下一篇

复合抗生素对生菜种子萌发及土壤酶活性的影响

郭佳祎1(), 于一雷2,3(), 李文彦2, 吕翠翠2, 刘超杰1   

  1. 1 北京农学院植物科学技术学院，北京 102206
    2 中国科学院雄安创新研究院（筹），河北雄安 071899
    3 地下水污染控制与修复教育部工程研究中心/北京师范大学，北京 100875
  • 收稿日期:2023-12-08 修回日期:2024-04-20 出版日期:2024-09-05 发布日期:2024-08-27
  • 通讯作者:
    于一雷，男，1981年出生，山东新泰人，研究员，博士，研究方向为污染生态学、同位素水文学研究。通信地址：071899 中国科学院雄安创新研究院（筹）安新园区，E-mail：。
  • 作者简介:

    郭佳祎，女，1999年出生，河北石家庄人，硕士研究生，研究方向为抗生素残留及风险评估研究。通信地址：102206 北京市昌平区回龙观镇北农路7号 植物科学技术学院，E-mail：。

  • 基金资助:
    地下水污染控制与修复教育部工程研究中心开放基金项目“雄安集约农田地下水氮素迁移转化的多元同位素示踪研究”(GW203312); 河北省重大科技成果转化专项“雄安创新研究院合作共建科技平台示范基地”(23267201Z)

Impact of Compound Antibiotics on Lettuce Seed Germination and Soil Enzyme Activity

GUO Jiayi1(), YU Yilei2,3(), LI Wenyan2, LV Cuicui2, LIU Chaojie1   

  1. 1 College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206
    2 Xiong’an Institute of Innovation, Chinese Academy of Sciences, Xiong’an, Hebei 071899
    3 Engineering Research Center of Groundwater Pollution Control and Remediation, Ministry of Education/ Beijing Normal University, Beijing 100875
  • Received:2023-12-08 Revised:2024-04-20 Published:2024-09-05 Online:2024-08-27

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摘要：

为探讨抗生素和灌溉水源变化对生菜、土壤的影响，以及其在培养第3、7 d时间上的变化，设计添加11种常见抗生素的复合污染水平(0、2.2、5.5、11 mg/kg)和2种不同灌溉水源的种子萌发及胁迫试验，并在发芽期间收集2次样品。结果表明，大部分生菜种子萌发指标（如种子的发芽势、发芽率、鲜重和苗长）在处理组与对照组间没有显著差异。在添加11 mg/kg浓度抗生素且灌溉水源为再生水时生菜种子的根长被显著抑制。采样期内（培养的第3、7 d），抗生素的添加和灌溉水源的变化没有扰乱生菜种子的抗氧化系统正常工作。培养第3~7 d，大多数处理组的土壤脲酶、土壤碱性磷酸酶活性有所上升，部分处理组与对照组之间存在显著差异，土壤酶活性相比植物萌发指标和植物酶活性对抗生素的添加更为敏感。

关键词: 抗生素, 复合污染, 生菜, 种子萌发, 土壤酶活性, 再生水, 生态风险

Abstract:

To investigate the effects of antibiotics and changes in irrigation water source on lettuce, soil and their changes in time on the 3rd and 7th day of incubation, seed germination and stress tests were designed with the addition of 11 common antibiotics at compound contamination levels (0, 2.2, 5.5 and 11 mg/kg) and two different irrigation water sources, and samples were collected twice during germination. The results showed that most of the lettuce seed germination indices (e.g., seed germination potential, germination percentage, fresh weight, and seedling length) were not significantly different in the treatment group compared to the control group. Root length of lettuce seeds was significantly inhibited when the concentration of addition of antibiotics was 11 mg/kg and the source of irrigation water was reclaimed water. During the sampling period (3rd and 7th day of incubation), the addition of antibiotics and the change in irrigation water source did not disturb the normal functioning of the antioxidant system of lettuce seeds. Soil urease and soil alkaline phosphatase activities increased in most of the treatment groups from the 3rd to the 7th day of incubation, and there was a significant difference between some treatment groups and the control group. Soil enzyme activities were more sensitive to the addition of antibiotics than plant germination indexes and plant enzyme activities.

Key words: antibiotics, composite pollution, lettuce, seed germination, antioxidant enzyme, reclaimed water, ecological risk