外源褪黑素对旱胁迫下花生种子萌发及抗性的影响

doi:10.11924/j.issn.1000-6850.casb2023-0613

中国农学通报 ›› 2024, Vol. 40 ›› Issue (18): 38-44.doi: 10.11924/j.issn.1000-6850.casb2023-0613

外源褪黑素对旱胁迫下花生种子萌发及抗性的影响

孙赛楠¹(), 苗鹏慧¹, 权月伟², 穆国俊¹, 郭丽果¹, 杨鑫雷¹()

¹ 华北作物改良与调控国家重点实验室/华北作物种质资源教育部重点实验室/河北省种质资源实验室/河北农业大学农学院，河北保定 071001
² 邯郸市农业科学院，河北邯郸 056000

收稿日期:2023-08-22 修回日期:2023-11-16 出版日期:2024-06-25 发布日期:2024-06-18
通讯作者:
杨鑫雷，男，1984年出生，河北涉县人，副教授，博士，研究方向：花生基因组学与分子育种。通信地址：071001 河北省保定市莲池区乐凯南大街2596号河北农业大学农学院花生育种室，Tel：13730227108，E-mail：peanut@hebau.edu.cn。
作者简介:
孙赛楠，女，1997年出生，河北张家口人，在读硕士研究生，研究方向：花生种质资源鉴定与利用。通信地址：071001 河北省保定市莲池区乐凯南大街2596号河北农业大学农学院花生育种室，Tel：18331310581，E-mail：Sweet_sun1008@163.com。
基金资助:
国家自然科学基金项目“花生侧枝角度性状的遗传分析和QTL定位”(31701459); 河北省青年拔尖人才资助项目(0602015); 河北省高等学校科学技术研究项目“基于Meta和连锁分析挖掘花生荚果大小QTL及候选基因”(ZD2022069); 河北农业大学科研发展基金计划项目“花生荚果大小相关性状QTL定位”(JY2021019); 华北作物改良与调控国家重点实验室自主课题项目“基于Meta和连锁分析挖掘花生产量性状QTL及候选基因”(NCCIR2022zz-6)

Effect of Exogenous Melatonin on Germination and Resistance Ability of Peanut Seeds Under Drought Stress

SUN Sainan¹(), MIAO Penghui¹, QUAN Yuewei², MU Guojun¹, GUO Liguo¹, YANG Xinlei¹()

¹ State Key Laboratory of North China for Crop Improvement and Regulation/North China Key Laboratory for Crop Germplasm Resources, Ministry of Education /Laboratory of Crop Germplasm Resources of Hebei Province/College of Agronomy, Hebei Agricultural University, Baoding, Hebei 071001
² Handan Academy of Agricultural Sciences, Handan, Hebei 056000

Received:2023-08-22 Revised:2023-11-16 Published:2024-06-25 Online:2024-06-18

摘要/Abstract

摘要：

本研究旨在探索外源褪黑素对干旱胁迫下花生种子萌发及抗性的影响。以干旱敏感型花生品种‘冀花5号’为试验材料，通过筛选种子萌发其适宜的PEG和褪黑素浓度，并设置空白水浸种(CK)，褪黑素浸种(T₁)，10%PEG-6000处理(T₂)和褪黑素浸种加10%PEG-6000处理(T₃)等4种处理，于种子萌发后进行抗氧化生理生化指标的测定。研究结果显示，模拟干旱的PEG-6000浓度为10%时，可以模拟干旱环境。同时，100 µmol/L的褪黑素浓度是促进花生种子萌发的最佳选择。进一步分析各处理花生种子胚根长度与粗度和抗氧化生理生化指标，发现干旱胁迫下施用100 µmol/L的褪黑素处理显著增强胚根生长势，并显著提高种子内SOD、POD和Pro的含量，且有效降低种子内MDA和H₂O₂的含量。综上所述，本研究表明外源褪黑素可以有效提高干旱胁迫下花生种子的萌发，为进一步探究干旱胁迫下褪黑素促进花生种子萌发的调控机制提供了理论依据。

关键词: 花生种子, 褪黑素, 干旱胁迫, 种子萌发, 外源褪黑素, 抗性, 抗氧化生理生化指标

Abstract:

The study aimed to explore the effect of exogenous melatonin on seed germination and resistance of peanut under drought stress. In this study, the drought-sensitive peanut variety ‘Jihua 5’ was used as the test material, and four treatments were set up, including blank water-soaked seed (CK), melatonin-soaked seed (T₁), 10% PEG-6000 treatment (T₂), and melatonin-soaked seed plus 10% PEG-6000 treatment (T₃), and four other treatments were used to determine the antioxidant physiological and biochemical indices after seed germination. The results showed that the 10% PEG-6000 was effective in simulating the drought environment. Meanwhile, melatonin concentration of 100 µmol/L was the best choice to promote peanut seed germination. Further analyzing the length and thickness of radicle and antioxidant physiological and biochemical indexes of peanut seeds under each treatment, we found that the application of 100 µmol/L melatonin under drought stress significantly enhanced the growth potential of radicle and increased the contents of SOD, POD and Pro, and effectively reduced the contents of MDA and H₂O₂ in peanut seeds. This study indicated that exogenous melatonin could effectively improve the germination of peanut seeds under drought stress, and provided a theoretical basis for further investigation of the regulatory mechanism of melatonin in promoting peanut seed germination under drought stress.

Key words: peanut seeds, melatonin, seed germination, drought stress, melatonin, resistance, antioxidant physiological and biochemical indicators

孙赛楠, 苗鹏慧, 权月伟, 穆国俊, 郭丽果, 杨鑫雷. 外源褪黑素对旱胁迫下花生种子萌发及抗性的影响[J]. 中国农学通报, 2024, 40(18): 38-44.

SUN Sainan, MIAO Penghui, QUAN Yuewei, MU Guojun, GUO Liguo, YANG Xinlei. Effect of Exogenous Melatonin on Germination and Resistance Ability of Peanut Seeds Under Drought Stress[J]. Chinese Agricultural Science Bulletin, 2024, 40(18): 38-44.

图/表 4

图1. PEG-6000浸种后花生种子发芽率

图2. 褪黑素浸种后花生种子发芽率

图3. 褪黑素处理对干旱胁迫下花生种子胚根长和粗的影响

图4. 不同处理对花生胚根的生理生化指标影响

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外源褪黑素对旱胁迫下花生种子萌发及抗性的影响

Effect of Exogenous Melatonin on Germination and Resistance Ability of Peanut Seeds Under Drought Stress

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