可控环境下培养基成分对大豆组培苗生根的影响

摘要/Abstract

摘要： 在植物组织培养过程中，温度、湿度、光照强度、CO2浓度等组培物理微环境对组培苗的生长发育有较大的影响。探讨在组培微环境控制的条件下，培养基成分对大豆（Glycine max L.）组培苗生根的影响以促进其生根并完善大豆再生体系。大豆外植体选用鲜重约85 mg的大豆组培单茎节移栽到添加了70 ml培养基的方型培养容器中。培养容器的容积为380 ml，其顶部留2个直径为10 mm的圆孔并覆盖高分子透气膜用来与容器外进行气体交换。试验区根据MS培养基中有糖、无糖和植物生长激素NAA与IBA 的不同浓度设置了六组，培育环境控制在温度23±1℃、湿度65±5%、光合有效光量子束密度70±9 μmol/(m2·s)、光周期16 h/d, CO2浓度未控制。在该可控环境下培育21d后，试验区S20-IBA1.0的大豆组培苗生根较好，净光合速率较高，显示出良好的生长趋势。试验表明：在该可控环境条件下利用添加20 g/L蔗糖和1.0 mg/L IBA的MS培养基有利于大豆组培苗生根，同时控制CO2浓度可期待大豆无糖组培生根能取得更好效果。

关键词: 盐胁迫, 盐胁迫, 牧草, 种子活力

Abstract: Micro culture environmental factors such as temperature, relative humidity, light intensity, and CO2 concentration are important to growth and development of plantlets in vitro. The aim of this study was to establish a culture process for soybean plantlet rooting under controlled environment. Single nodal soybean (Glycine max L.) explants each with 85 mg fresh mass were planted at two gas-permeable filtered culture vessels containing 70 ml medium and cultured at air temperature of 23±1℃, relative humidity of 65±5%, photosynthetic photon flux of 70±9 μmol/(m2·s), photoperiod of 16 h/d, and without CO2 control for 21 days. The medium components were MS basic ingredient and B5 medium vitamins appending six combinations of sugar (0, 20 g/L) and hormone (0.5 and 1.0 mg/L IBA; 0.5 and 1.0 mg/L NAA). The thickset rooting and growth of soybean plantlets were shown in all treatments and the treatment with 20 g/L sugar and 1.0 mg/L IBA showed greater growth variables and net photosynthetic rate than other treatments. The culture process using photoautotrophic micropropagation could be superior for soybean plantlet rooting compared to conventional tissue culture process.

中图分类号:

鲍顺淑,贺冬仙. 可控环境下培养基成分对大豆组培苗生根的影响[J]. 中国农学通报, 2006, 22(11): 60-60.

Bao Shunshu, He Dongxian. Effects of Medium Components on Rooting of Soybean Plantlet under Controlled Environment[J]. Chinese Agricultural Science Bulletin, 2006, 22(11): 60-60.

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