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中国农学通报

中国农学通报 ›› 2007, Vol. 23 ›› Issue (1): 258-258.

所属专题： 生物技术

• 目次 • 上一篇    下一篇

桔全爪螨抗辛硫磷品系的选育及多功能氧化酶的特性研究

陈志永,冉 春,张 玲,王进军   

  • 出版日期:2007-01-05 发布日期:2007-01-05

Resistance Selection to Phoxim and Properties of Mixed Function Oxidase in Panonychus citri

Chen Zhiyong, Ran Chun, Zhang Ling, Wang Jinjun   

  • Online:2007-01-05 Published:2007-01-05

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摘要： 在实验室内对桔全爪螨进行辛硫磷抗性选育，选育12代后抗性上升了18.6倍。比较抗辛硫磷品系和相对敏感品系体内多功能氧化酶的酶学特性及辛硫磷和增效醚（PBO）对该酶的抑制作用，结果表明，桔全爪螨抗性品系多功能氧化酶的活性、比活力和米氏常数（Km）均显著高于敏感品系。辛硫磷对敏感品系体内MFO的抑制效果显著强于抗性品系，且MFO活性抑制率与药剂抑制浓度之间呈显著的线性关系。以PBO作用于桔全爪螨，相对敏感品系和抗性品系MFO的活力均受到抑制，但对抗性品系的抑制效果强于敏感品系。研究结果说明桔全爪螨对辛硫磷的抗性形成与多功能氧化酶的活力的升高有关。

关键词: 魔芋, 魔芋, 组培苗, 生长调节物质, 瓶外生根, 组织培养

Abstract: The resistance to Phoxim in Panonychus citri was selected in laboratory. After 12 generations of selections, Panonychus citri have developed 18.6-fold resistance to phoxim. By the Comparison of the properties of mixed function oxidase (MFO) between the resistant and susceptible strains and the inhibition of phoxim and PBO to MFO of P. citri, it was found that the activities, specific activity and the Michaelis–Menten constant (Km) of MFO in the resistant strain were significantly higher than those in susceptible strain, and the inhibition of phoxim to MFO in susceptible strain was significantly greater than that in resistant strain. There was a significantly linear relationship between the inhibition rate of MFO activity and the inhibition concentration of the Phoxim. However, the inhibition of PBO to MFO in resistant strain was significantly higher than that in susceptible strain. It was suggested that the development of resistance to phoxim in P. citri might be related to the rise of the MFO activity.

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