微生物和酶学技术在烟草发酵中的应用及展望

摘要/Abstract

摘要： 综述了微生物和酶学技术在烟草发酵中的应用及其研究进展，重点阐述了陈化烟叶表面微生物区系与优势菌种的筛选、分离和鉴定；外源有益微生物协调与改善陈化烟叶化学组分，提高烟叶香气量，缩短烟叶陈化周期；以及添加外源生物活性酶降解烟叶中淀粉、蛋白质、纤维素等大分子物质等方面的研究现状，分析了目前该领域研究中存在的问题，认为将现代分子生物学技术与传统的方法相结合来深入研究烟叶发酵机理及其增香技术仍是今后研究的关键。

关键词: 兰州百合, 兰州百合, 鳞茎, 离体繁殖

Abstract: The application and progress of the technology of microbe and enzyme during tobacco fermentation were reviewed, especially expatiated on the researching condition of isolating and identifying of the microflora on tobacco leaf during aging, utilizing some useful microbes, which can well balance the chemical components, increase aroma of tobacco leaf and decrease ferment time, reducing the content of starch, protein, cellulose and so on in tobacco leaves by adding biological enzyme, analyzed the problems in the researches, think that it is the key that combining the technology of biomolecular with the traditional method to study the theory of tobacco aging and the technology of promoting flavor in tobacco.

中图分类号:

TS414

赵铭钦,李芳芳. 微生物和酶学技术在烟草发酵中的应用及展望[J]. 中国农学通报, 2007, 23(1): 314-314.

Zhao Mingqin, Li Fangfang. The Application and Prospect of the Technology of Microbe and Enzyme during Tobacco Leaf Fermentation[J]. Chinese Agricultural Science Bulletin, 2007, 23(1): 314-314.

参考文献

[1] 赵铭钦,齐伟城,邱立友,等.烟草发酵增质剂对烤烟发酵质量的影响.河南农业科学,1998,(3):7-9.
[2] 邱立友,赵铭钦,岳雪梅,等.自然发酵烤烟叶面微生物区系的分离鉴定.烟草科技,2000,(3):14-17.
[3] 陈福星,王磊.烟叶微生物发酵的探讨.湖南微生物学通讯,1990,(2):37-39.
[4] Tamayo A I, Cancho F G. Microbiology of the fermentation of Spanish tobaccos. International congress of Microbiology,1953,6:48-50.
[5] English C F, Bell E J, Berter A J. Isolation of thermopiles from roadleaf tobacco and effect of pure culture inoculation on cigar
aroma and mildness, Appl.Microbio,1967,15:117-119.
[6] 马林.利用生物技术改变烟叶化学组分提高其吸食品质和安全性的研究.郑州工程学院学报,2001,22(3):40-42.
[7] 罗家基,朱子高,罗毅,等.微生物在烟叶发酵过程中的作用.烟草科技,1998,(1):6.
[8] 朱大恒,陈锐,陈再根,等.烤烟自然醇化与人工发酵过程中微生物变化及其与酶活性关系的研究,中国烟草学报,2001,7(2):26-30.
[9] 程彪,贾涛,刘立全,等.烟草工业中微生物和酶作用的控制与利用.烟草科技,1999,(3):8-11.
[10] Reid J J, Gribbons M F, Haley D E. The fermentation of cigar-leaf tobacco as influenced by the addition of yeast.J.Agric.Redearch,1944,69:373-381.
[11] Tamayo A I, Cancho F G. Microbiology of the fermentation of Spanish tobaccos. International congress of Microbiology, 1953,6:48-50.
[12] Pounds J R, Lucas G B. Thermophilic Fungi of tobacco. North Carolina Agricultural Experiment station, Tech, Bull, 1972,211.
[13] 谢和,秦京,王亮,等.优势微生物在烤烟人工发酵过程中的作用,贵州农学院丛刊,1990,(1):95-107.
[14] 陈福星,王磊,莫湘涛,等.烟叶微生物发酵的探讨.微生物通讯,1990,(2):37-38.
[15] 韩锦峰,朱大恒,刘卫群,等.陈化发酵期间烤烟叶面微生物活性及其应用研究.中国烟草科学,1997,(4):13-14.
[16] 赵铭钦,邱立友,张维群,等.陈化期间烤烟叶片中生物活性变化的研究.华中农业大学学报,2000,19(6):537-542.
[17] Reid J J, Mckinstry D W，Haley E E．Studies on the fermentation of tobacco I. The microflora of cured and fermenting cigar-leaf tobacco. Pennsylvania Agricultural Experiment Station Bulletin,1933,(356):1-17.
[18] Giovannozzi-Sermanni G. Study of the fermentation of tobacco.Coresta.1960, (1):3319.
[19] Giovannozzi-Sermanni G. Study of the fermentation of tobacco.Coresta.,1962, (1):5621.
[20] Dawso R F. Talk on the cigarette filler symposium. Kansas: Philip Morris Tobacco Company,1965.
[21] Koller J B C. Der tobak in naturwiss enschaflicher, land wirtschaff licher and technischer bezichung , Augsburg,1958.
[22] Koiwai A, Matsumoto T, Nishida K, et al. Studies on the fermentation of tobacco. Tobacco science,1970,14:103-105.
[23] 徐洁,张修国,郑小嘎,等.烤烟诱香微生物及其制剂的研究初选.云南烟草,2003,(1):25-29.
[24] 大恒,韩锦峰,周御风,等.利用产香微生物发酵生产烟用香料技术及其应用.烟草科技, 1997, (1):30-31.
[25] 周瑾,李雪梅,许传坤,等.利用微生物发酵改良烤烟碎片品质的研究.烟草科技,2002,(6):3-5.
[26] 朱大恒,韩锦峰.利用优势微生物抑制烟叶霉变的研究.中国烟草学报,1999,5(2):23-24.
[27] V. L. Geiss. Control and use of microbes in tobacco product manufacturing. R.A.T.S. 1989, 15: 182-209.
[28] Wada E. and Yamasaki K. Degradation of nicotine by soil bacteria.Journ.Am.Chem.1954, (76): 155.
[29] Frankenburg W. G. and Vaitekunas A. A. Chemical studies on nicotine degradation by microorganisms derived from the surface of tobacco seeds. coreata 1957,(1):0034.
[30] Giovannozzi-Sermanni G. A new species of corynebacterium causing nicotine degradation. Coresta 1957, (3):0605.
[31] Casida T. E. Jr. Bacterial oxidation of nicotine. Formation of γ-aminobutyric(AB) acid. J. Bacterial. 1958, (75):474-479
[32] Wada E. Microbial degradation of the tobacco alkoloids and some related compounds. Arch. Biochem. Biophys.1958:72(1),145-162.
[33] Giovannozzi-Sermanni G. Arthrobacter nicotianae a new type of Arthrobacter causing nicotine degradation. Coresta,1959,(3):2595.
[34] Lawrence E. Gravely, Louisville Ky, Vernon L. Geiss, et al. Process for reduction of nitrate and nicotine content of tobacco by microbial treatment. US Patent:4557280,1978 .
[35] Civilini Marcello, Domenis Cristina, Sebastianutto Nerina, et al. Nicotine decontamination of tobacco agro-industrial waste and its degradation by microorganisms waste manage. Res.,1997,15(4):349-358.
[36] 王革,王颖琦,李松,等.微生物发酵烟叶降解尼古丁、蛋白质.烟草科学研究,2001,(2):66-68.
[37] 周冀衡,朱小平,王彦亭.烟草生理与生物化学.中国科学技术大学出版社,1996,463-487.
[38] 韩锦峰,朱大恒,杨素勤,等.不同陈化时期烤烟几种酶活性及相关化学分析的分析.中国烟草科学,1999,(1):1-2.
[39] C. F. Bailey and A. W. Petre. Progress Report. Philip Morris& Company Ltd, Dec 31,1937,10.
[40] 张立昌.烟叶酶处理的作用效果.烟草科技,2001,(4):7-9.
[41] 任军林,刘振宇.施加高活性微生物转化酶提高卷烟感官质量的实验.烟草科技,2000,(4):9-10.
[42] 姚光明.降低烟叶中蛋白质含量的研究.烟草科技,2000,(9): 6-8.