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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (20): 44-50.doi: 10.11924/j.issn.1000-6850.casb16020103

所属专题： 生物技术

• 植物保护 农药 • 上一篇    下一篇

深绿木霉T2发酵液蛋白提取物TraT2A诱导兰州百合抗灰霉病研究

韩 亮,梁巧兰,周其宇   

  1. (甘肃农业大学草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心,兰州 730070）
  • 收稿日期:2016-02-28 修回日期:2016-06-24 接受日期:2016-03-25 出版日期:2016-07-15 发布日期:2016-07-15
  • 通讯作者: 梁巧兰
  • 基金资助:
    甘肃省财政厅项目“深绿木霉T2蛋白激发子TraT2A分离纯化及诱导抗病作用机理研究”[甘财教2013(116)]。

Resistance of Lanzhou Lily to Botrytis cinerea Induced by Protein Extract TraT2AFrom Trichoderma atroviride T2 Fermentation Liquid

Han Liang, Liang Qiaolan, Zhou Qiyu   

  1. (Pratacultural College, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem of MOE/Pratacultural Engineering Laboratory of Gansu Province/Sino-U.S. Centers for Grazing Land Ecosystem Sustainability, Lanzhou 730070)
  • Received:2016-02-28 Revised:2016-06-24 Accepted:2016-03-25 Online:2016-07-15 Published:2016-07-15

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摘要： 为了探讨深绿木霉T2发酵液蛋白提取物TraT2A诱导兰州百合对灰霉病菌产生抗性，采用不同浓度的TraT2A测定了对百合灰霉病菌的抑菌率和诱导抗病作用，其后用TraT2A的100倍液喷雾处理兰州百合，48 h后接种灰霉菌，研究其诱导抗病机理。结果表明：10倍、100倍TraT2A对百合灰霉菌有一定的抑制作用和诱导抗病作用，100倍液的抑菌率最小，仅为1.16%，但其诱导抗病效果最大，为84.77%。诱导处理TraT2A+接种灰霉菌能显著提高兰州百合叶片中GLU、PAL、POD、PPO活性及蜡质含量并促进胼胝质的积累，其中该处理后的POD、PPO活性和蜡质含量在测定时间内均高于处理TraT2A、接种灰霉菌和CK，GLU活性除第3天外，其余时间也高于其他3个处理。GLU、PAL活性在第7天达到最大值，分别为284.28、2.93 U，比其他3个处理分别高出0.09、0.31、0.46倍和0.31、0.19、0.47倍；POD、PPO活性和蜡质含量在第1天达到最大值，分别为1.59 U、0.63 U、14.48 mg/g，比其他3个处理分别高出0.04、0.29、0.43倍，0.12、0.32、0.67倍和0.09、0.06、0.99倍；胼胝质积累也均高于其他各处理。说明TraT2A诱导处理能够激活百合防御体系，使百合产生抗病性。

关键词: 水资源脆弱性, 水资源脆弱性, 动态演变, 南方丘陵区, GIS, RS

Abstract: In order to induce the resistance of Lanzhou lily against Botrytis cinerea, different concentrations of protein extract TraT2A from Trichoderma atroviride T2 fermentation liquid were added to the culture medium. The antibacterial rate and the induced resistant effect to Botrytis cinerea were investigated. To study the induced disease resistant mechanism, the 100 times diluent of TraT2A was sprayed to healthy Lanzhou lily, and Botrytis cinerea was inoculated after 48 hours (TraT2A IB), and the other three treatments were TraT2A, IB(inoculating Botrytis cinerea ) and CK (the control group). The results indicated that the 10 times and 100 times diluent of TraT2A had antibacterial effect on Botrytis cinerea, and the antibacterial rate of 100 times diluent of TraT2A was the minimum, which was only 1.16%, but the induced resistant effect was the maximum, which reached 84.77%. The treatment of TraT2A Botrytis cinerea significantly improved the activities of GLU, PAL, POD, PPO, wax content and the accumulation of callose. And the activities of POD, PPO and the wax content were higher than that of other treatments. The activity of GLU was higher than that of other treatments except in the third day. The activities of GLU and PAL reached the maximum in the seventh day, which were 284.28 and 2.93 U, respectively, and was 0.09, 0.31, 0.46 times and 0.31, 0.19, 0.47 times higher than that of TraT2A, IB and CK treatment, respectively. Meanwhile, the activities of POD and PPO and the max content all reached the maximum in the first day, which were 1.59 U, 0.63 U and 14.48 mg/g, respectively. The activity of POD in the treatment of TraT2A IB was 0.04, 0.29, 0.43 times higher than that of the other three treatments, the activity of PPO was 0.12, 0.32, 0.67 times and the wax content was 0.09, 0.06, 0.99 times higher than that of the other three treatments. And the accumulation of callose was also higher than that of the other three treatments. The results indicated that the treatment of TraT2A IB could activate the defense system of lily and induce disease resistance.

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