Abstract: To investigate the change characteristics of peroxidase and esterase isozyme, POD and EST isozymes in leaves and roots during different growth stages of eight chewing cane varities were studied by plumb plate of polyarclamide gel electrophoresis. The cluster analysis showed that: (1) the zymogram patterns genetic similarity coefficient in different varieties exist difference, especially in between Badila and local varieties.(2) Dendrograms of POD and EST zymogram patterns of roots and leaves in different growth stages exist difference. (3) the significantly correlations were observed in the zymogram patterns of leaves during different periods. The result showed leaf is a active spot of expression of POD and EST, and could be used as a more stable and suitable sampling sites.