桃儿七不同外植体愈伤组织诱导研究

doi:10.11924/j.issn.1000-6850.casb15100033

中国农学通报 ›› 2015, Vol. 31 ›› Issue (36): 150-155.doi: 10.11924/j.issn.1000-6850.casb15100033

所属专题：生物技术

桃儿七不同外植体愈伤组织诱导研究

郭生虎,朱永兴,关雅静

(宁夏农林科学院农业生物技术研究中心,银川 750002）

收稿日期:2015-10-12 修回日期:2015-11-04 接受日期:2015-11-18 出版日期:2015-12-30 发布日期:2015-12-30
通讯作者: 郭生虎
基金资助:
宁夏农林科学院自主研发项目“濒危中药材桃儿七离体扩繁技术研究”(NKYG-14-06)。

Callus Induction of Different Explants of Sinopodophyllum emodi (Wall.) Ying

Guo Shenghu, Zhu Yongxing, Guan Yajing

(Agricultural Biotechnology Center, Ningxia Academy of Agriculture and Forestry Science, Yinchuan 750002)

Received:2015-10-12 Revised:2015-11-04 Accepted:2015-11-18 Online:2015-12-30 Published:2015-12-30

摘要/Abstract

摘要： 旨在建立一种桃儿七愈伤组织诱导培养体系，筛选出最佳诱导培养基和最适外植体。以MS为基本培养基，采用正交试验法研究6-BA、2,4-D和TDZ 3个因素及其组合对桃儿七成熟叶片、叶柄和茎段愈伤组织诱导率的影响。研究结果表明：3种外植体均可诱导出愈伤组织，叶片愈伤组织诱导率最高，达44.3%，其次是叶柄，达24.7%，茎的愈伤组织诱导率为18.1%，最佳愈伤组织诱导培养基为MS+ 6-BA 1.0 mg/L+TDZ 0.1 mg/L+2,4-D 1.5mg/L，细胞分裂素6-BA和TDZ联合使用有利于提高愈伤组织诱导率并改善愈伤组织质地。与茎段和叶片所得愈伤组织相比，以叶柄为外植体获得的愈伤组织质地较疏松，呈明显的黄绿色颗粒状，更适合进一步细胞培养和诱导分化。本研究建立了一种高效的桃儿七愈伤组织诱导培养体系，为桃儿七体细胞胚的培养提供了材料。

关键词: 梨花, 梨花, 幼果, 霜冻, 指标, 临界温度

Abstract: The study aims to establish a technology system for callus induction and culture of Sinopodophyllum emodi (Wall.) Ying, and to select the optimum medium and explants. The authors adopted the orthogonal experimental design to study the effects of MS basic media with 6-BA (6-Benzlaminopurine), 2,4-Dichlorophenoxyacetic acid and TDZ (Thioridazine) and their combinations on induction rate of the explants of leaf and petiole and stem from Sinopodophyllum emodi (Wall.) Ying. The results showed that the callus could be induced from three explants and the leaf was the best explant with a high induction rate of 44.3% and the rate of petiole was 24.7%, the rate of stem was 18.1%. The best suitable medium was MS 6-BA 1.0 mg/L TDZ 0.1 mg/L 2,4-D 1.5mg/L. Combining cytokines of 6-BA and TDZ was beneficial to improve the induction rate and the quality of callus. Compared with the callus of leaf and stem, the loose granular and yellowish green callus from petiole was fit for further inducing differentiation and cell culture. An efficient technology system for callus induction and culture of Sinopodophyllum emodi (Wall.) Ying was established, and it provided the materials for culture of somatic embryogenesis.

中图分类号:

S566.1

郭生虎,朱永兴,关雅静. 桃儿七不同外植体愈伤组织诱导研究[J]. 中国农学通报, 2015, 31(36): 150-155.

Guo Shenghu,Zhu Yongxing and Guan Yajing. Callus Induction of Different Explants of Sinopodophyllum emodi (Wall.) Ying[J]. Chinese Agricultural Science Bulletin, 2015, 31(36): 150-155.

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桃儿七不同外植体愈伤组织诱导研究

Callus Induction of Different Explants of Sinopodophyllum emodi (Wall.) Ying

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