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中国农学通报

中国农学通报 ›› 2007, Vol. 23 ›› Issue (5): 71-71.

所属专题： 小麦

• 生物技术科学 • 上一篇    下一篇

小麦单倍体育种中培养基的研究

蔡东明,吉万全,任志龙,王秋英,王长有,张 宏,王亚娟   

  • 出版日期:2007-05-05 发布日期:2007-05-05

Studies on Culture Medium of Wheat Haploid Breeding

Cai Dongming, Ji Wanquan, Ren Zhilong, Wang Qiuying, Wang Changyou, Zhang Hong, Wang Yajuan   

  • Online:2007-05-05 Published:2007-05-05

PDF (PC)

635

摘要： 小麦单倍体育种中培养基直接影响幼苗产生率，为了找到一种适宜小麦幼胚生长的培养基,对16份F1单交组合幼胚在8种培养基上的生长发育状况进行了研究，8种培养基的幼苗平均产生率分别为32.71%、41.48%、47.15%、53.59%、57.98%、62.05%、53.26%和42.21%。培养基①、②、③、⑧的幼苗平均产生率较低，培养基④、⑤、⑥、⑦的较高，其中培养基⑥(1/2MS+0.2 mg/L IAA+0.2 mg/L 6-BAP+2%蔗糖+7.5g/L琼脂 PH=5.8)的幼苗产生率为62.05%，且幼芽分化明显，根系弯曲至瓶底，叶色深绿，较其他7种培养基培养效果最好。

Abstract: Culture medium affects seedling production of wheat haploid. Growth and development of 16 single F1 on eight kinds of medium were studied in order to find a kind of the optimal culture medium for growth of wheat young embryo. Average seedling production frequency of 8 eight kinds of medium were 32.71%, 41.48%, 47.15%, 53.59%, 57.98%, 62.05%, 53.26% and 42.21%, respectively. Average seedling production frequency of culture medium ①, ②, ③ and ⑧ were lower and that of ④, ⑤, ⑥ and ⑦ were higher. Seedling production frequency of the medium ⑥, (1/2MS+0.2 mg/L IAA+0.2 mg/L6-BAP+2% sucrose +7.5g/L agarose, PH=5.8), was 62.05%. The young germnation differentiation of the medium ⑥ was obvious, and roots curved to the bottle bottom and leaf was deeper green. The results showed that the culture effect of the medium ⑥ was better than that of the other.

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