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中国农学通报 ›› 2007, Vol. 23 ›› Issue (5): 67-67.

所属专题: 油料作物 园艺

• 生物技术科学 • 上一篇    下一篇

甘蓝型油菜银染MRNA差别显示条件的建立及优化

秦 岭, ,张春雷   

  • 出版日期:2007-05-05 发布日期:2007-05-05

Establishment and Optimization of Sliver Staining Differential Display of Brassica napus L.

Qin Ling, , Zhang Chunlei   

  • Online:2007-05-05 Published:2007-05-05

摘要: mRNA 差异显示技术是分析差异表达基因的强有力的工具。本实验针对油菜差别显示体系筛选优化,最终获得了差别显示体系。研究结果为:RNA模板在2μg,反转录产物用量为1.5μl时,可以扩增出较多且清晰的条带;PCR退火温度在40℃扩增条带清晰。该体系用于甘蓝型油菜低磷胁迫前后基因差异表达研究稳定可靠。

关键词: 纤维素分解菌, 纤维素分解菌, 秸秆, 酶活力

Abstract: mRNA differential display is a powerful instrument to indentify differentially expressed genes.This experiment is designed to optimize a differential display with silver staining of oilseed, and a optimized differential display system is obtained. The results showed :Many and clear bands were observed ,When the amount of RNA used was about 2μg and the amount of reverse transcription products used was 1.5μl. The annealing temperature on DDRT-PCR Amplification was 40℃,which produced many cDNA band. This system which was used for the analysis of gene expression under low phosphorus condition in Brassica napus L. is stable and reliable.