植物多酚氧化酶研究综述

摘要/Abstract

摘要： 多酚氧化酶(Polyphenol Oxidase 简称PPO)是一类广泛分布于植物体中的一种铜结合酶。它是由核基因编码、多基因控制，在细胞质中合成，通过一定的方式转运至质体内而成为具酶活性的形式，对农产品品质形成有重要影响。介绍了多酚氧化酶在植物体内的存在和定位、分子结构、生理功能及调控措施等方面近年来的研究成果。

关键词: 银杏, 银杏, 药用价值, 组织培养

Abstract: Polyphenol oxidase (PPO) is a ubiquitous copper metalloprotein in many plants, PPO is encoded by multiple nucleicgenes, synthesized in cytoplasm , transferred into plastids in some forms , and then they become of enzyme activity. It is believed to be the primary factor for the quality of many crops. In this article, the research advances on existence, localization, molecular structure, physiological function, expression and regulation of Polyphenol Oxidase.

中图分类号:

Q945

代丽,宫长荣,史霖,陈付军,巩培智. 植物多酚氧化酶研究综述[J]. 中国农学通报, 2007, 23(6): 312-312.

Dai Li, Gong Changrong, Shi Lin, Chen Fujun, Gong Peizhi. Polyphenol Oxidase in Plants[J]. Chinese Agricultural Science Bulletin, 2007, 23(6): 312-312.

参考文献

[1] 李荣林,方辉遂.一个世纪以来对多酚氧化酶研究的进展[J].福建茶叶,1997,(4):10-14.
[2] 谢春艳,宾金华,陈兆平.多酚氧化酶及其生理功能[J].生物学通报,1999,34(6):11-13.
[3] Piyada Thipyapong, et al. Differential Expression and turnover of the tomato polyphenol oxidase gene family during vegetative and reproductive development [J].Plant Physiol,1997,(113):707-718.
[4] 吴红梅,萧慧,刘刚.多酚氧化酶的研究进展[J].茶业通报,2004,26(2):62-64.
[5] 武德传,徐晓燕,董召荣.烟草多酚氧化酶及其与烟草品质关系的研究进展[J].安徽农业科,2006,34(7):1381-1383.
[6] Taneja S R, Abrol Y P, Sachar R C. Modulation of oidiphenolase and monophenolase enzymes during wheat grain development [J].Cereal Chem,1974,(51):457-450.
[7] Kruger J E. Changes in the polyphnol oxidase of wheat during kernel growth and maturation [J].Cereal Chem, 1976,(53):201-213.
[8] P W Thygesen, I B Dry, S P Robinson. Polyphenol oxidase in potato[J]. Plant Physiol,1995,(109):525-531.
[9] Hunt M D, Eannetta N T ,Yu H, et al. cDNA cloning and expression of potato polyphenol oxidase[J]. Plant Molecular Biology ,1993,(21):59-68.
[10] Dry IB, Robinson Sally MNewman SP, Eannetta NT, et al. Molecular cloning and charaterization of grape berry polyphenol oxidase gene [J ]. Plant Mol Biol, 1994 ,(26):495-502.
[11] Chizuyo Kato, Ikuzo Uritani, Ryoyasu Saijo,et al.Cellular localization of particulate-bound polyphenol oxidase in tea leaves [J]. Plant Cell Physiol,1976,(17):1045-1052.
[12] 黄明,彭世清.植物多酚氧化酶研究进展[J].广西师范大学学报,1998,16(2):65-69.
[13] Kowalski SP, Eannetta NT, Hirzel AT, et al.Purification and characterization of polyphenol oxidase from glandular trichomes of Solanum berthaultii [J]. Plant Physiol,1992,(100):677-684.
[14] Newman SM , Eannetta NT ,Yu H, et al.Organisation of the tomato polyphenol oxidase gene family [J]. Plant Molecular Biology,1993,(21):1053-1058.
[15] Soderhall I, Soderhall K. Purification of prophenol oxidase from daucus carota cell cultures [J]. Phytochem,1989,28(7):1805-1808.
[16] Demeke T, and Morris C F. Molecular characterization of wheat polyphenol oxidase (PPO) [J]. Therotioal and Applied Genetics ,2002,(104):813-818.
[17] Chevalier T, Rigal D, Mbeghie Ambeguie D, et al. Molecular cloning and characterization of apricot fruit polyphenol oxidase [J]. Plant Physiol, 1999,(119):1261-1269.
[18] Sally M Newman, Nancy T Eannetta, Haifeng Yu, et al. Organisation of the tomato polyphenol oxidase gene family [J]. Plant Molecular Biology, 1993,21(6):1035-1051.
[19] Hind G, Marshak DR, Coughlan SJ. Spinach thylakoid polyphenol oxidase: Cloning, Charaterization, and relation to a Putative protein kinase[J]. Biochem,1995,34:8157-8164.
[20] Cary JW, Lax AR, Flurkey WH. Cloning and characterization of cDNA coding for Vicia faba polyphenol oxidase[J]. Plant Mol Bio ,1992,(20):245-253.
[21] Tamar Shahar, Nava Hennig, Tamar Gutfinger, et.al. The Tomato 66.3-kD Polyphenoloxidase Gene: Molecular Identification and Developmental Expression[J]. The Plant Cell, 1992,4:135-147.
[22] 赵东,刘祖生,等.茶树多酚氧化酶基因的克隆及其序列比较[J].茶叶科学,2001,21(2):94-98.
[23] Shi C,Dai Y, Xu X,et al.The purification of polyphenol oxidase from tobacco[J].Protein Expression and Purification,2002, 24(1):51-55.
[24] Sommer A, Ne′eman E, Steffens JC. Import, targeting, and processing of aplant polyphenol oxidase[J]. Plant Physiology, 1994,105:1301-1311.
[25] 王曼玲,胡中立,周明全,等.植物多酚氧化酶的研究进展[J].植物学通报,2005,22(2):215-222.
[26] Paul S, Colin B, Simon P, et al. Molecular cloning and characterization of banana fruit polyphenol oxidase [J]. Planta, 2001,(213):748-757.
[27] 王全华,王秀峰.番茄抗病基因工程育种研究进展[J].山东农业大学学报,2003,34(4):600-604.
[28] Piyada Thipyapong, Michelle D Hunt , John C Steffens. Antisense downregulation of polyphenol oxidase results in enhanced disease susceptibility[J]. Planta, 2004,220(1):103-117.
[29] 李保聚,李凤云.黄瓜不同抗性品种感染黑星病菌后过氧化物酶和多酚氧化酶的变化[J].中国农业科学,1998,31(1):86-88.
[30] 石雪晖,王淑英,吴艳纯,等.葡萄叶片中单宁、木质素、PPO 活性与抗黑痘病的关系[J].中外葡萄与葡萄酒,1997,(4):8-11.
[31] 张建军,李祥,候明生.小麦品种对梭条斑花叶病毒病抗病特性的研究Ⅱ抗病性与植株内多酚氧化酶活性、过氧化物酶活性及其同工酶酶谱的关系[J].华中农业大学学报,1996,15(5):420-425.
[32] 谢文华,谢大森.棱角丝瓜不同品种对霜霉病抗性的相关研究[J].华南农业大学学报,1999,20(2):28-31.
[33] 张红梅,黄丹枫,丁明,等.不同苗龄接穗的西瓜嫁接体愈合过程中的3种酶活性变化[J].植物生理学通讯,2005,41(3):302-304.
[34] 史俊燕,樊金拴,严江.酚类物质及其相关酶对核桃嫁接成活的影响[J].西北林学院学报,2005,20(1):80-83.
[35] 陈桂信,潘东明,赖钟雄,等.园艺植物多酚氧化酶的分子生物学研究进展[J].江西农业大学学报,2003,25(1):107-110.
[36] 宫长荣,周义和,杨焕文.烤烟三段式烘烤导论[M].北京:科学出版社,2006:143-145.
[37] 宫长荣,李巍,司辉.下部烟叶采收时间对上部叶生理生化变化及烤后质量的影响[J].烟草科技／栽培与调制,2003,(194):36-41.
[38] 吕作新,刘好宝,刘彩萍,等.烟叶烘烤过程中的酶促棕色化反应及其调控途径[J].中国烟草科学,1997,(2):19-21.
[39] AKEHURST B C.Tobacco[M].London and York, 1981:616-618.
[40] Christian W, B Bachem, Gert-Jan Speckmann, et al. Antisense Expression of Polyphenol Oxidase Genes Inhibits Enzymatic Browning in Potato[J]. Tubers Bio Technology, 1994,(12):1101-1105.
[41] Chris Coetzer, Dennis Corsini, Steve Love. Control of Enzymatic Browning in Potato (Solanum tuberosum L.) by Sense and Antisense RNA from Tomato Polyphenol Oxidase [J]. J.Agric. Food Chem, 2001,49(2):652-657.
[42] 林伯年,吴晓梅,胡军.梨(Pyrus pyr if olia Naka i) 和莲藕(N elumbo nucif era Gearte)组织中多酚氧化酶特性及褐变控制[J].浙江农业大学学报,1997,23(S):63-67.
[43] 吴锦程,夏海玲,唐朝晖,等.白肉枇杷多酚氧化酶酶学特性研究[J].中国农学通报,2005,21(11):96-115.
[44] 雷东锋,蒋大宗,王一理.烟草中多酚氧化酶的生理生化特征及其活性控制的研究[J].西安交通大学学报,2003,37(12):39-44.
[45] 汪敏,郑师章.凤眼莲体内多酚氧化酶的生理生化特性[J].复旦学报,1994,33(2):157-164.
[46] 尹建雄,卢红.烟草中多酚化合物及多酚氧化酶研究进展[J].广西农业科学,2005,36(3):284-286.
[47] 肖厚荣,徐小龙,解永树,等. pH诱导烟草多酚氧化酶二级结构变化的光谱学研究[J].化学物理学报2004,17(2):196-200.