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中国农学通报

中国农学通报 ›› 2007, Vol. 23 ›› Issue (9): 0-0.

• 生物技术科学 •    下一篇

魔芋试管微球茎繁殖技术研究

顾玉成,吴金平,,宋志红,向发云,曾祥国,胡中立   

  • 出版日期:2007-09-05 发布日期:2007-09-05

Study on Progenitive Technology of Test-tube Micro- bulbodium of Amorphophallus konjac

Gu Yucheng, Wu Jinping,, Song Zhihong, Xiang Fayun, Zeng Xiangguo, Hu Zhongli   

  • Online:2007-09-05 Published:2007-09-05

摘要： 针对目前魔芋生产存在种芋繁殖系数低的问题，研究可用于大规模生产魔芋试管微球茎的诱导技术。实验通过3种不同方法切割魔芋愈伤组织分化的幼苗，分别置入不同激素配比的4种培养基中培养。培养基MS+ 6-BA2.0 mg/L +NAA0.5 mg/L +蔗糖6%和MS+ 6-BA0.8mg/L +NAA0.5 mg/L +蔗糖6%适合愈伤组织的形成和不定芽的分化。培养基1/2MS+ NAA0.08mg/L +蔗糖6%和1/2MS+ IBA0.8 mg/L +蔗糖6%适合叶柄基部带愈伤组织的幼苗生根培养，其中1/2MS+ NAA0.08 mg/L +蔗糖6%的培养基适合叶柄基部带少量愈伤组织的幼苗诱导微球茎，将形成的微球茎，从叶柄基部撇断，可看到微球茎上有芽点。通过研究发现魔芋试管微球茎的形成不仅与培养基的组成有关，还与叶柄基部的切割方式有关

关键词: 拟南芥, 拟南芥, 磷饥饿, 突变体, 筛选

Abstract: The question of the low propagation coefficient in the production of Amorphophallus at present, the research may use in the large scale production induction technology for test-tube micro-bulbodium. This experiment cut to the seedling for callus differentiation through three different methods, separately setting in the different hormone allocated proportion four kind of culture medium. Results showed that callus was induced and differentiated in media consisted of MS+ 6-BA2.0 mg/L +NAA0.5 mg/L + 6% sucrose and MS+ 6-BA0.8mg/L +NAA0.5 mg/L + 6% sucrose. 1/2MS+ NAA0.08 mg/L + 6% sucrose and 1/2MS+ IBA0.8 mg/L + 6% sucrose were fit for the base of the stem with some callus for rooting, and 1/2MS+ NAA0.08 mg/L + 6% sucrose was suitable for the base of the stem with little callus to induce micro-bulbodium, breaking from the base of stem , there is the bud spot as the micro-bulbodium. It was discovered that the formation of test-tube Micro- bulbodium not only with the culture medium composition related, but also with the way of cutting for the seedling base

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