猪圆环病毒1型ORF2基因的克隆与序列分析

摘要/Abstract

摘要： 根据GenBank中猪圆环病毒1型（PCV1）ORF2基因序列，设计一对引物，应用PCR方法从PK-15细胞和疑似患断奶仔猪多系统衰竭综合症（PMWS）的仔猪组织病料中分别扩增出PCV1 ORF2全基因（702bp）。将此基因片段克隆入pMD 18-T载体，筛选获得重组质粒pMD-PCV1-ORF2并对其测序，结果表明：所克隆的PK-15来源和组织病料来源的ORF2基因的同源性为99.4%，二者与Genbank上发表的PCV1 ORF2基因的同源性介于97.3% ~ 99.9%，与PCV2-ORF2基因同源性介于67.9% ~ 68.4%，二者编码的蛋白在几个功能区域比较保守；抗原表位预测表明PCV1 ORF2编码的蛋白具有良好的免疫原性

关键词: 新海14, 新海14, 密度, 生育特性

Abstract: The ORF2 genome was amplified from PK-15 cells and tissues of piglets suffered from PMWS respectively using the primers designed according to the OFR2 sequence published in Genbank. Then the resultant PCR products were cloned into pMD18-T vector. The nucleotide sequence data of PCV1-ORF2 gene was analyzed by comparing with that of other PCV1-ORF2 and PCV2-ORF2 gene sequences. The results indicated that the nucleotide homology of ORF2 gene cloned from PK-15 cells and pig tissues was 99.4%, the homology of all selected PCV1-ORF2 genes was 97.3%~99.9%, and the homology of PCV1-ORF2 and PCV2-ORF2 was 67.9%~68.4%. Meanwhile, several conserved founctional domains were discovered in PCV1-ORF2 gene, and the forecast of antigenicity indicated the protein translated by PCV1-ORF2 gene having well immunogenicity.

中图分类号:

S852.659.2

靳玉芬,郝贵增,何宏轩. 猪圆环病毒1型ORF2基因的克隆与序列分析[J]. 中国农学通报, 2007, 23(9): 3-3.

Jin Yufen, Hao Guizeng, He Hongxuan. Cloning and Sequencing Analysis of ORF2 Gene of Porcine Circovirus 1[J]. Chinese Agricultural Science Bulletin, 2007, 23(9): 3-3.

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