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中国农学通报 ›› 2007, Vol. 23 ›› Issue (11): 179-179.

所属专题: 园艺

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油茶优良无性系花粉形态和生活力研究初报

彭邵锋,,陈永忠,张日清,李党训,陆 佳   

  • 出版日期:2007-11-05 发布日期:2007-11-05

Preliminary Studies on Pollen Morphology and Pollen Viability of Superior Clones of Camellia oleifera

Peng Shaofeng,, Chen Yongzhong, Zhang Riqing, Li Dangxun, Lu Jia   

  • Online:2007-11-05 Published:2007-11-05

摘要: 【研究目的】了解油茶优良无性系花粉形态及花粉生活力,探讨花粉生活力的测定方法,并对花粉生活力进行分级。【方法】以新鲜花粉为试验材料,观察花粉形态,采用人工直接授粉法、TTC染色法和过氧化物酶法测定其花粉生活力。花粉生活力分级采用聚类分析和实践经验相结合的方法。【结果】在光学显微镜下油茶花粉颜色呈黄色或淡黄色,形状从平面角度观察呈椭圆形、三裂圆形、圆形等,花粉大小为44.1μm×29.2μm;不同品系的油茶花粉生活力差别很大。【结论】油茶花粉形状绝大部分为椭圆形,花粉大小属于中等。花粉生活力可分为5级,其测定必须结合多种方法,而TTC法是较为理想的快速测定法。

关键词: 桃金娘, 桃金娘, 药用, 引种, 罗浮山。

Abstract: 【OBJECTIVE】In order to study on the pollen morphology and pollen viability of the superior clones of camellia oleifera, and the determination method and classification of pollen viability.【METHOD】Some fresh pollen of Camellia oleifera was employed as the experimental material to carry on the studies on pollen morphology and determination method of pollen viability. Artificial fertilization method, TTC staining and peroxidase settling process were conducted to determine the pollen viability. Classification for pollen viability was completed by cluster analysis combined with experience.【RESULTS】Through optical microscope, the color of Camellia oleifera pollen was yellow or light yellow, the shape of pollen are ellipse, 3-crack round and round, and the size was 44.1μm×29.2μm. As to the pollen viability, there was significant difference between different clones.【CONCLUSION】The shape of most of camellia oleifera pollen was ellipse, and the size of them was moderate-sized. The pollen viability can be classified into five groups, but it should be combined with many testing methods to determine pollen viability before drawing a conclusion, TTC staining method was more suitable for determining pollen viability quickly.

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