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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (10): 148-150.

所属专题： 生物技术

• 农学 农业基础科学 • 上一篇    下一篇

不同激素浓度对柠条茎段组织培养的影响

霍晓兰,闫敏,张强,孟全业   

  • 收稿日期:2008-10-17 修回日期:2008-11-04 出版日期:2009-05-20 发布日期:2009-05-20

Effects Of Different Plant Hormone Concentrations On StemTissue Culture Of Caragan microphylla

  • Received:2008-10-17 Revised:2008-11-04 Online:2009-05-20 Published:2009-05-20

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摘要： 以小叶锦鸡儿茎段为外植体，MS为基本培养基，设计不同浓度的IAA、IBA、NAA、6-BA等单因子和不同比例的细胞分裂素组合试验。结果表明：在芽的诱导增殖过程中， MS+NAA1.5mg/L+6-BA0.5mg/L的培养基效果好，诱导率为87.3%。生根培养过程中， MS+IAA5mg/L的培养基效果好。

关键词: 播期, 播期, 密度, 氮肥运筹方式, 产量

Abstract: Stalk segments of Caragan microphylla Lam were cultured as explants on medium supplemented with plant hormone in different concentrations.The results showed that Murashige and Skoog(MS) medium supplemented with NAA 1.5mg/L,6-BA0.5mg/L was the most effective medium for the differentiation and multiplication of the shoots. The induction rate was 87.3%. The optimum rooting medium was MS+IAA5mg/L.