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中国农学通报 ›› 2009, Vol. 25 ›› Issue (3): 265-270.

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

一株高效降解氯氰菊酯细菌的分离鉴定及降解特性

张松柏,张德咏,罗香文,成飞雪,罗源华,刘勇   

  • 收稿日期:2008-11-27 修回日期:2008-12-13 出版日期:2009-02-05 发布日期:2009-02-05

Biodegradation of Cypermethrin by a Newly Bacterial Isolate, Kurthia sp. LF-1

  • Received:2008-11-27 Revised:2008-12-13 Online:2009-02-05 Published:2009-02-05

摘要: 从长期使用氯氰菊酯的土壤中筛选分离出三株优势菌,编号为LF-1、LF-2和LF-3。选择对氯氰菊酯降解潜力最高的菌株LF-1进行鉴定和降解特性研究。根据生理生化特征以及16SrDNA序列同源性比对将LF-1初步鉴定为kurthia sp.;降解特性结果表明,LF-1降解氯氰菊酯最适pH和温度分别为7,35℃;在最佳降解条件下培养8 d,对100 mg/L氯氰菊酯降解率达80.15%,LF-1还能降解甲氰菊酯、联苯菊酯等菊酯类农药。经SDS或EB消除质粒后,LF-1降解氯氰菊酯的能力丧失, 明该菌降解基因可能位于质粒DNA。

关键词: 唇形科, 唇形科, 药用植物, 种质资源, 区系, 河南

Abstract: In order to obtain the strains of effectively degrading cypermethrin, the bioremediation bacteria were enriched and isolated from cypermethrin contaminated soil. Three predominant bacteria were obtained, and named as LF-1, LF-2 and LF-3. Of these, the LF-1 was selected for further study for biggest potential of cypermethrin degradation. LF-1 was identified as a member of kurthia sp. based on its biochemical characteristics and homology of 16SrDNA sequence (Genbank Accession No. EU677769). The cypermethrin quantity was analyzed accurately by GC with µECD. The results showed 35℃, pH 7 were the optimum degradation conditions, respectively. LF-1 could degrade cypermethrin up to 80.15% in a concentration of 100 mg/L within 8 d, and also degrade fenpropathrin, bifenthrin effectively. The plasmid cured strain could not degrade cypermethrin, which indicated that the degradation gene was probably involved in plasmid. The degradation characters showed the potential to be used in a bioremediation application in agriculture.