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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (4): 69-71.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

基于改良SDS法的杏基因组DNA提取

杨 丽,张俊环,孙浩元,王玉柱   

  • 收稿日期:2008-02-29 修回日期:2008-03-11 出版日期:2008-04-05 发布日期:2008-04-05

Study on Apricot Genomic DNA Extraction by Modified SDS Method

Yang Li, Zhang Junhuan, Sun Haoyuan, Wang Yuzhu   

  • Received:2008-02-29 Revised:2008-03-11 Online:2008-04-05 Published:2008-04-05

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摘要： 为探讨改良SDS法是否适用于杏基因组DNA提取及提取液适宜浓度，以6个品种杏叶片为试材提取DNA，提取液设0.5%、1%、2%三个浓度梯度。检测结果表明，改良SDS法完全适用于杏叶片基因组DNA的提取，3种浓度的提取液均可得到质量较高的DNA， 且0.5%为改良SDS法提取杏叶片基因组DNA的适宜提取液浓度。

关键词: 白魔芋, 白魔芋, 种质资源, 遗传分析, RAPD

Abstract: To probe the adaptability of modified SDS method in apricot genomic DNA extraction and the optimal concentration, the genomic DNA samples of 6 apricot cultivars were isolated by 0.5%, 1%, 2%SDS extracted buffer. The results showed that good quality DNA would be obtained by modified SDS method and the 0.5% is better.