阜平大枣无芽茎段再生不定芽的初步研究

中国农学通报 ›› 2009, Vol. 25 ›› Issue (13): 150-153.

阜平大枣无芽茎段再生不定芽的初步研究

周小丽,彭建营,段红喜

收稿日期:2009-04-02 修回日期:2009-05-07 出版日期:2009-07-05 发布日期:2009-07-05

Preliminary Study on Adventitious Bud Regeneration from in Vitro Non-bud Stem of Ziziphus jujuba Mill.‘Fupingdazao’

Zhou Xiaoli, Peng Jianying, Duan Hongxi

Received:2009-04-02 Revised:2009-05-07 Online:2009-07-05 Published:2009-07-05

摘要/Abstract

摘要： 以MS为基本培养基,幼嫩的阜平大枣无芽茎段为试材,研究了灭菌时间、暗培养、以及植物生长调节剂对无芽茎段诱导不定芽再生的影响。结果表明：无芽茎段以灭菌8-10分钟为宜，暗培养21天最佳，在MS基本培养基上附加6-BA 1.0mg.L-1，，IBA 0.1mg.L-1再生率和平均出芽数显著高于其它处理，分别为85.42%和 3.23，而且不定芽经培养后生长成枣头一次枝。

关键词: 槐山羊, 槐山羊, 屠宰性能, 肉用品质

Abstract: The factors affecting adventitious bud regeneration from in vitro non-bud stem of Ziziphus jujuba Mill.‘Fupingdazao’ in MS media were studied, which included sterilization time, dark incubation time and plant growth regulator. The results showed that 8-10 minutes of sterilization time for young non-bud stem was suitable; 21 days of dark culture was optimal ;The best combination of plant growth regulators was confirmed on MS+6-BA 1.0mg.L-1+ IBA 0.1mg.L-1，on which regeneration rate and Adventitious buds per stem were 85.42% and 3.23 respectively, they were significantly higher than other treatment, and the adventitious buds could become developmental branch.

周小丽,彭建营,段红喜. 阜平大枣无芽茎段再生不定芽的初步研究[J]. 中国农学通报, 2009, 25(13): 150-153.

Zhou Xiaoli, Peng Jianying, Duan Hongxi. Preliminary Study on Adventitious Bud Regeneration from in Vitro Non-bud Stem of Ziziphus jujuba Mill.‘Fupingdazao’[J]. Chinese Agricultural Science Bulletin, 2009, 25(13): 150-153.

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