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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (18): 348-351.

所属专题： 生物技术

• 植物保护 农药 • 上一篇    下一篇

黄绿木霉T1010适宜的发酵条件分析

陈建爱 王同燕 王未名 李志国 裘纪莹 杜方岭   

  • 收稿日期:2009-04-16 修回日期:2009-05-26 出版日期:2009-09-20 发布日期:2009-09-20
  • 基金资助:

    山东省良种工程项目

Trichoderma aureoviride 1010 Was Cultured with the Proper Condition

wang tongyan   

  • Received:2009-04-16 Revised:2009-05-26 Online:2009-09-20 Published:2009-09-20

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587

摘要：

木霉广泛存在于土壤、根际及其它基质中，木霉对植物土传病具有拮抗作用。优化生防因子黄绿木霉（Trichoderma aureoviride）突变体T1010发酵条件是扩大生产、大面积应用的前提。本试验通过测定黄绿木霉T1010的菌丝生长和分生孢子生长，优化了培养条件，确定最适宜种子培养用PD培养基，摇瓶培养，生长时间为3天，此时菌丝生长量为5.75g/ml；最经济适宜扩大培养基为玉米15g/L，豆粉5g/L，摇瓶培养时间为3天，此时菌丝生长量为5.83g/ml，发酵罐培养时间2.5天为宜；最适宜经济的固体发酵培养基为麦麸：醋渣：水=2：1：3.3，尿素为0.3%，发酵温度为26 ± 2°C，发酵产物孢子量为2.46×1010cfu/g。本文研究确定的发酵工艺参数为高效率、低成本、标准化生产黄绿木霉制剂提供了科学依据。

关键词: 黑光灯, 黑光灯, 趋光性昆虫, 种群动态

Abstract:

Trichoderma spp. is fungus that is highly interactive in root, soil and foliar environments. They can biocontrol against a wide range of plant-soilborne-pathogen. Trichoderma aureoviride 1010 (T1010) strain was cultured in economical proper media will be important to be applied in agriculture. Hyphae were cultured in PD liquid for 3 days and the weight of hyphae was 5.75g/ml. The second growth was cultured in corn and soy-bean mixture media for 3 days, the weight of hyphae was 5.83g/ml. The economical proper solid media was wheat bran: vinegar residue: H2O=2:1:3.3. The mixing urea was 0.3%. T1010 grew at 26 ± 2°C. The number of T1010 spores was 2.46×1010cfu/g. This fermentation process parameter in this test will put high-effective, lower-cost, standardization to the proof of Trichoderma preparation.