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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (18): 352-355.

所属专题： 水稻

• 植物保护 农药 • 上一篇    下一篇

水稻条纹病毒楚雄分离物一个重组RNA序列分析

程文金,吴祖建,谢联辉   

  • 收稿日期:2009-04-20 修回日期:2009-05-11 出版日期:2009-09-20 发布日期:2009-09-20
  • 基金资助:

    农业部公益性行业（农业）科研专项

A recombinant RNA from Chuxiong isolate of Rice stripe virus(RSV) was sequenced

  • Received:2009-04-20 Revised:2009-05-11 Online:2009-09-20 Published:2009-09-20

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摘要：

测序鉴定水稻条纹病毒（Rice stripe virus, RSV）云南楚雄分离物（YCX07）的重组片段YCX07R。该片段由RSV RNA2的3'端序列与RNA3的3'端序列重组而成，具有RSV基因组结构特征，采取双义编码策略，即在正、负链的5'端分别存在一个阅读框，编码两个重组蛋白YCX07R-5P、YCX07R-3P。推测重组系由RNA流产性复制引起或重组的两个片段通过重组点直接联结形成。

关键词: 畜禽, 畜禽, 线粒体DNA, 多态性, 分子进化, 起源

Abstract:

A recombinant RNA of one isolate of Rice stripe virus(RSV) from Chuxiong(YCX07), Yunnan Province, China, was sequenced. The RNA was recombined from the 3' segments of RNA2 and RNA3 of RSV. The recombinant RNA uses ambisense coding strategy and encodes two proteins, namely YCX07R-5P and YCX07R-3P. The two proteins located on the 5' end of the plus and minus strand, respectively. This is similar to the four RNAs of RSV. It was discussed that the RNA was recombined from abortion replication or linked by two recombinant fragments through the recombinant site directly.