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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (20): 267-271.

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

溶藻细菌W-04的筛选及溶藻效果初探

王海玉1,赵 芳1,彭 谦2,孙珮石1   

  • 收稿日期:2009-04-22 修回日期:2009-04-27 出版日期:2009-10-20 发布日期:2009-10-20
  • 基金资助:

    云南大学校级科研项目

Isolation of an algae-constraining bacteria strain W-04 and its effects on the growth of M. aeruginosa Kütz

  • Received:2009-04-22 Revised:2009-04-27 Online:2009-10-20 Published:2009-10-20

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摘要：

利用筛选果胶分解菌的培养基从滇池水样中筛选到的1株能利用果胶作为唯一碳源的红色细菌（编号为W—04），将它与铜绿微囊藻（M. aeruginosa Kütz）共培养，发现藻细胞数量与加入的菌液及无菌滤液的量呈负相关关系，菌藻液体（带菌或无菌滤液）体积比为10/100的试验组藻细胞第8天时残存数为0，连续培养30天叶绿素测定结果为0，经显微观察藻细胞数也为0，表明藻体包括叶绿素已完全降解，无再度繁殖能力.

关键词: 杂交油葵, 杂交油葵, 盐胁迫, 耐盐性, 鉴定, 生长抑制

Abstract:

A red bacterium named w-04 that could use pectin as the only carbon source was isolated from Dian-chi Lake. When cultivated with M. aeruginosa Kütz, the number of M. aeruginosa Kütz cells showed negative dependence on the amount of bacteria added. The number of M. aeruginosa Kütz cells changed periodically with the time of cultivation. The duration of the period was about 6 days. With the experimental group in which the ratio of liquid volume of bacteria to M. aeruginosa Kütz was 10 to 100, the viable cells of M. aeruginosa Kütz droped to 0 after 8-day cultivation. No chlorophyll was detected in the culture after 30-day continuous cultivation, indicating the M. aeruginosa Kütz cells, as well as chlorophyll, were broken down and could not propagate anymore.