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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (19): 26-31.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

四种真菌基因组编码的细胞壁降解酶生物信息学分析

田新生 吴培星   

  • 收稿日期:2009-05-13 修回日期:2009-06-08 出版日期:2009-10-05 发布日期:2009-10-05
  • 基金资助:

    国家“十一五”高技术研究发展计划（863）项目;甘肃省农业生物技术研究与应用开发项目

Bioinformatic analysis of plant cell wall degrading enzymes in the genomes of four different fugi

  • Received:2009-05-13 Revised:2009-06-08 Online:2009-10-05 Published:2009-10-05

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954

摘要：

本文对两种病原性真菌[稻瘟病菌(M.grisea)、小麦赤霉病菌(F.graminearum)]和两种非病原性真菌[粗糙脉孢菌(N.crassa)、构巢曲霉(A.nidulans)]基因组编码的植物细胞壁降解酶（PCWDEs）进行了广泛的生物信息学研究。植物病原性真菌可以通过分泌PCWDEs降解宿主植物的细胞壁。PCWDEs能够穿过植物细胞壁的天然屏障，并生成一些有利于真菌生长的简单小分子物质。因此，PCWDEs在植物的致病过程中可能扮演着十分重要的角色。本研究首先通过BLAST（Basic Local Alignment Search tool）结合人工二次筛选确定了四种真菌基因组编码的不同植物细胞壁降解酶（PCWDEs）的数目；然后，利用SignalP来判断PCWDEs能否被分泌到细胞外。通过在基因组水平上对四种病原性和非病原性真菌基因组编码的PCWDEs的比较，我们可以发现不同真菌之间确实存在着一些显著的生物学差异。

关键词: 60Coγ射线, 60Coγ射线, 大花高代, 半致死剂量, 矮化

Abstract:

This paper presents a comprehensive study on identifying the number of genes encoding different classes of plant cell wall degrading enzymes in the genomes of pathogenic and nonpathogenic fungi. Plant pathogenic fungi secrete extracellular enzymes that are able to degrade the plant cell walls of their host plants. These plant cell wall degrading enzymes are so necessary for penetration of the cell wall barriers, as well as for generation of simple molecules that can be assimilated for growth. Initially, BLAST was exerted to compare pathogenic (M.grisea, F.graminearum) and non?pathogenic fungi (N.crassa, A.nidulans) and the top hits of them were acquired. Then SignalP were performed to verify whether these PCWDEs would be secreted by the fungi.Consequently, we conclude that some significant biological differences of PCWDEs can be discovered between pathogenic and nonpathogenic fungi.