欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (24): 62-67.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

风信子DFR基因全长cDNA的克隆及序列分析

祁银燕,刘雅莉,李 莉,王跃进   

  • 收稿日期:2009-08-24 修回日期:2009-09-30 出版日期:2009-12-20 发布日期:2009-12-20
  • 基金资助:

    国家自然科学基金（30871734）

Cloning and Sequencing Full-length cDNA of DFR gene in Hyacinthus

  • Received:2009-08-24 Revised:2009-09-30 Online:2009-12-20 Published:2009-12-20

PDF (PC)

1009

摘要：

以蓝色风信子完全露出蓝色的花蕾为材料,利用RT-PCR和RACE技术，获得风信子DFR基因的全长cDNA。该cDNA全长1252 bp ,开放阅读框为1098 bp ,编码366个氨基酸。 Blast搜索结果显示,风信子DFR基因核苷酸序列与其它植物已报道的DFR基因具有66 %～77 % 的相似性,氨基酸序列有62 %～73 %的相似性。聚类分析表明, 最先与风信子聚类合并的是鸢尾，其次与其它单子叶植物聚类，最后与双子叶植物聚类。

关键词: 耐甲氧西林葡萄球菌, 耐甲氧西林葡萄球菌, mecA基因, PCR, 核酸探针

Abstract:

A total length DFR gene was cloned with RT-PCR and RACE technology from flower bud with blue petals of Hyacinthus orientalis L.. Hyacinthus DFR cDNA was 1252 bp in length.Within the f ull-length cDNA , a clear open reading frame (ORF) was 1098 nucleotides ,coding 366 amino acids. The result s of homologous analysis in GenBank demonstrated that the sequence had 66 %～77 % identity on the nucleotide sequence and 62 %～73% identity on the deduced amino acid sequence. The results of phylogenetic analysis showed that DFR from Hyacinthus orientalis and from Iris x hollandica clustered together firstly ,and then came those from other monocotyledon, dicotyledon came subsequently.