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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (23): 327-330.

• 林学 园艺 园林 • 上一篇    下一篇

大花蕙兰与春剑杂交原球茎增殖及分化研究

王丰妍 李承秀   

  • 收稿日期:2009-08-28 修回日期:2009-09-04 出版日期:2009-12-05 发布日期:2009-12-05
  • 基金资助:
    泰安市大学生科技创新项目

Study on Propagation and Differentiation of PLB of Cymbidium hybridum ×Cymbidium Longibracteatum

  • Received:2009-08-28 Revised:2009-09-04 Online:2009-12-05 Published:2009-12-05

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摘要： 【研究目的】对大花蕙兰品种‘绿宝石’与春剑杂交原球茎增殖与分化进行研究。【方法】设置不同培养基、6-BA 和 NAA以及不同添加物对杂交兰原球茎增殖分化的进行探讨。【结果】KC培养基为最佳培养基；6-BA对原球茎的增殖影响不大, NAA对增殖影响较明显当浓度为0.2mg/L原球茎增殖效果最好；KC+6-BA2.0 mg/L+ NAA 0.5 mg/L对原球茎的分化效果最好；【结论】在培养基中加15mL/L香蕉汁最有利于杂交兰原球茎的增殖。

关键词: 黔苦2号, 黔苦2号, 春播, 籽粒产量, 农艺措施

Abstract: 【OBJECTIVE】The research aimed to study the propagation and differentiation of PLB of Cymbidium hybridum‘Queen Emeraldus’and C. longibracteatum. 【METHOD】 Four factors of medium, 6-BA, NAA, and additive were designed to study the effects on differentiation and differentiation of PLB of Cymbidium hybridum ×C. longibracteatum .【RESULTS】KC medium was more effective than others. There was no difference with different concentrations of 6-BA. NAA influenced the propagation of PLB directly. NAA0.2mg/L was most favorable to the propagation of PLB. The best differentiation medium was KC+6-BA2.0mg/L+NAA0.5mg/L. 【CONCLUSION】 The medium supplemented with 15mL/L banana was most beneficial to the Propagation of PLB.