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中国农学通报 ›› 2017, Vol. 33 ›› Issue (34): 26-31.doi: 10.11924/j.issn.1000-6850.casb16110073

所属专题: 生物技术 油料作物 农业气象

• 农学 农业基础科学 • 上一篇    下一篇

干旱胁迫下外源ABA对鼓粒期大豆产量及氮代谢关键酶活性的影响

屈春媛   

  1. 黑龙江八一农垦大学农学院
  • 收稿日期:2016-11-15 修回日期:2017-11-26 接受日期:2016-12-23 出版日期:2017-12-07 发布日期:2017-12-07
  • 通讯作者: 屈春媛
  • 基金资助:
    黑龙江八一农垦大学研究生创新项目“干旱胁迫下外源ABA对鼓粒期大豆氮素积累和转移的调控效应研究”(YJSCX2016-Y07);国家自 然科学基金“外源ABA调控干旱胁迫下鼓粒期大豆氮素积累和转移的机制研究”(204134008);国家自然科学基金项目“锰对大豆抗旱耐盐能力促进 的分子机制研究”(31401332);国家科技支撑计划“大豆玉米轮作与经济高效施肥技术体系研究”(2014BAD11B01-02);国家现代农业产业技术体系项 目(CARS-04-01A);黑龙江省垦区科研项目“大豆“减投增效”生产栽培技术的试验与示范”(HNK135-02-06)。

Effect of Exogenous ABA on Yield and Key Enzyme Activities of Nitrogen Metabolism of Soybean Under Drought Stress

  • Received:2016-11-15 Revised:2017-11-26 Accepted:2016-12-23 Online:2017-12-07 Published:2017-12-07

摘要: 通过研究鼓粒期外源脱落酸(ABA)在干旱胁迫条件下与氮素同化关键酶的关系,为大豆产量在实际生产中提供理论依据。采用2 种不同类型的大豆为材料,在15%聚乙二醇(PEG-6000)模拟干旱条件下,于鼓粒期进行干旱胁迫处理(PEG)和干旱胁迫+喷施脱落酸处理(PEG+ABA),研究外源ABA在干旱胁迫下对大豆硝酸还原酶(NR)、谷氨酰胺合成酶(GS)和谷氨酸合成酶(GOGAT)活性的调控效应。试验结果表明,从鼓粒始期到鼓粒末期,在正常供水(CK)情况下,供试2 个品种大豆叶片中NR和GS活性急剧降低,GOGAT活性呈先下降再上升趋势。与对照相比,PEG处理均降低叶片中NR、GS、GOGAT活性,在鼓粒后21 天与CK、PEG+ABA处理产生显著差异,导致大豆籽粒产量较低。PEG+ABA处理叶片NR、GS和GOGAT活性高于PEG处理,差异显著;在鼓粒后28 天和35 天,其活性大小急剧上升,有利于大豆籽粒产量的形成。由此说明,外源ABA可以缓解逆境胁迫带来的伤害,同时可以促进氮代谢反应,从而控制叶片的衰老速度,提高籽粒产量。

关键词: 普兰店市, 普兰店市, 气温, 趋势分析, 变化周期

Abstract: The main purpose of this paper was to study the relationship between key enzymes of nitrogen assimilation and exogenous abscisic acid(ABA), provide theory basis for soybean production in the actual production under drought stress of soybean during seed-filling period. The drought resistance was studied in two different cultivars at seedling stage under a simulated drought stress condition by using 15 % concentration of polyethylene glycol (PEG) in vitro in order to investigate and analyze the effect of exogenous ABA under drought stress on activities of NR、GS and GOGAT activities of soybean leaf during seed-filling period. The results showed that NR and GS activity of soybean leaves were decreased sharply during the seed-filling period, GOGAT activity first decreased and then increased under the condition of normal water supply. Compared with the control, PEG treatment decreased the activity of NR、GS and GOGAT. It produced significant differences compared with CK and PEG ABA treatment in the 21 d after beginning of seed-filling to lead the lower yield of soybean. PEG ABA treatment increased the activity of NR、GS and GOGAT, they were higher than PEG treatment and they were significantly different; their activity size were increased sharply during 28 d and 35 d after beginning of seed-filling to promote the formation of soybean seed.The results indicated that exogenous hormones improved soybean tolerance under drought stress and promote the nitrogen metabolic reaction, thereby slow down the senescence of soybean leaves and provide the grain yield.