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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (3): 129-133.

• 林学 园艺 园林 • 上一篇    下一篇

湿加松外植体消毒及初始培养基筛选研究

常金财1,2,宗亦臣1,郑勇奇1,白淑兰2   

  • 收稿日期:2009-09-01 修回日期:2009-09-15 出版日期:2010-02-05 发布日期:2010-02-05
  • 基金资助:

    科技部林业科技支撑计划“高产优质多抗松杉新品种选育”专题;中央级公益性科研院所专项资金项目“杂交松无性系快繁技术研究”;国家科技基础条件平台“外来树种种质资源标准化整理整合与共享”

Study on Explants Sterilization and Initial Culture Medium Selection of Pinus elliottii × P. caribaea

  • Received:2009-09-01 Revised:2009-09-15 Online:2010-02-05 Published:2010-02-05

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摘要：

摘 要：本文以2年生澳大利亚种源湿加松茎尖为试材，研究了不同消毒程序防控外植体污染的效果，得出最佳消毒程序为茎尖清水冲洗5min， 用含表面活化剂吐温-20的无菌水冲洗1min，70%乙醇消毒10s，0.1%HgCl2浸泡6.5min，无菌水冲洗5遍。以MS、1/2MS、GD、DCR、WPM、N6等6种针叶树常用培养基，细胞分裂素6-BA、生长素NAA进行湿加松基本培养基的筛选，调查芽的诱导、增殖、伸长和生根的情况。筛选出湿加松初始培养基为GD+BA(0.1－1.0mg/L)+NAA(0.1－0.4 mg/L)。

关键词: 农民, 农民, 培训需求, 对策

Abstract:

This study intended to establish a culture system that would induce explant sterilization and initial medium. The stem of hybrid pine was 2 years old, which seeds were made of Pinus elliottii across Pinus caribaea of Australia. The results showed that the best way of explants sterilization was as follows: stem was washed in water for 5 minutes, in sterile water with tween-20 for 1 minute, in 70% ethanol for 10 seconds, in 0.1% HgCl2 for 6.5 minutes, and washed in sterile water for 5 times. Selection of the initial culture medium was with MS, 1/2 MS, GD, DCR , WPM and N6, which were common culture mediums on pine. 6-BA and NAA were also added to the selected medium. Inductivity, proliferation, elongation and root rate of bud were as the evaluating indicator. The better culture medium of hybrid pine was GD+BA(0.1－1.0mg/L)+NAA(0.1－0.4 mg/L).