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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (5): 189-192.

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

无籽西瓜茎段组织培养与嫁接育苗技术研究

王闯1,李中勇2,刘敏1,豆惠敏1,毛志泉3   

  • 收稿日期:2009-09-29 修回日期:2009-10-29 出版日期:2010-03-05 发布日期:2010-03-05

Studies on the Tissue Culture of the Stems and Vitro-grafting Technology in Seedless Watermelon

  • Received:2009-09-29 Revised:2009-10-29 Online:2010-03-05 Published:2010-03-05

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摘要：

以无籽西瓜(Citrullus lantus)墨童无菌苗的茎段为外植体，对组织培养与嫁接育苗技术研究进行了研究。结果表明：不同消毒剂对种胚萌发有一定影响,其中消毒剂二氧化氯250mg/L 处理有利于种胚萌芽,萌发率达82.5%；子叶芽诱导的最佳培养基配方为1/2MS +IAA0.2 mg/L+蔗糖30g/L+琼脂7g/L；不同激素浓度对芽丛增殖和单芽生根也有一定影响,其中MS +6-BA0.4 mg/L +IAA0.2 mg/L +蔗糖30g/L+琼脂7g/L继代培养增殖率最高；MS+NAA0.5mg/L生根率最高(96.2%)。

关键词: 频振式杀虫灯, 频振式杀虫灯, 灯光诱杀, 综合防治, 有机茶园

Abstract:

In this paper,studies on tissue culture and grafting of vegetable motong in seedless watermelon are carried though. The results showed that different disinfectors have definite effectson bourgeonof watermelon embryo.In which,disinfectorof Chlorine Dioxide (250 mg/L)usedto treat watermelon embryo have advantages in germinationof watermelon embryo.The most ouitable mediumf or buds induetion from eotyledon of Citrulluslanatus was 1/2MS +IAA0.2 mg/L+sucrose 30g/L + agar 7g/L;Different consistencies of incretionhave also definite effects on multiplication of acervate buds ,and the succeedculture medium of MS +6-6-BA0.44 mg/L +IAA0.2 mg/L + sucrose 30g/L +agar 7g/L can obstain the most multiplicating rate;The result of studyon different consistencies of NAA in rooting of single bud showed that the culture medium of MS+NAA0.5 mg/L have th emost rooting rate(96. 2%).