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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (8): 1-6.

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 •    下一篇

咖啡因和维生素E对小尾寒羊精液冷冻保存的影响

崔旭1,罗桂河2,李卫东3,李复煌4,孟丹娜2,吕建伟1,穆笛1,倪和民1   

  • 收稿日期:2009-10-14 修回日期:2009-11-16 出版日期:2010-04-20 发布日期:2010-04-20
  • 基金资助:

    北京市教育委员会科技发展计划重点项目

Effect of different concentrations of caffeine, VE on the freezing semen of small-tailed Han sheep

Cui Xu1, Luo Guihe2, Li Weidong3, Li Fuhuang4, Meng Danna2, Lü Jianwei1, Mu Di1,   

  • Received:2009-10-14 Revised:2009-11-16 Online:2010-04-20 Published:2010-04-20

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摘要：

摘 要：【目的】研究冷冻稀释液中分别添加不同浓度的咖啡因和维生素E后对小尾寒羊精液冷冻保存的影响。【方法】将不同浓度的咖啡因和维生素E分别添加于冷冻前的小尾寒羊精液稀释液中，用精子活率、顶体完整率和畸形率，作为判定精子冷冻品质的指标，初步确定两种稀释液添加成分对小尾寒羊精液品质的影响。【结果】当添加维生素E的浓度为0.4mg/ml时，冷冻后的精子活率较高，畸形率较低，且精子顶体完整率也较高；添加5mg/ml咖啡因能显著提高精子的解冻后活率，其活率为0.35，对精子畸形率和顶体完整率无显著影响。【结论】在冷冻精液稀释液中添加适当浓度维生素E可以减缓冷冻对精子的伤害，提高小尾寒羊冷冻精子活率，改善冷冻精液的品质；当添加5mg/ml的咖啡因于稀释液中冷冻时，解冻后精液活率显著提高，但不影响精子畸形率和顶体完整率。

关键词: 半边旗, 半边旗, 生物学性状, 抗癌机理, 繁殖技术, 资源保护利用

Abstract:

Abstract: 【Objective】This study was aimed to investigate the effect of different concentrations of caffeine, vitamin E (VE) on the freezing semen of small tailed Han sheep. 【Method】The different concentrations of caffeine and VE were added to the pre-frozen sheep semen diluent in small-tailed Han sheep, then the motility rate of their post-thawed semen, the percentage of sperms with intact acrosome, and the percentage of their abnormality, were respectively estimated to detect the quality of their frozen semen. 【Result】The results were shown as following: While the concentration of VE was 0.4mg/ml, after freezing and thawing, the livability rate of the semen from small tailed Han sheep is high, whereas their abnormality rate is low, but the apical body percentage of sperm head is still high. Meanwhile, adding with 5mg/ml caffeine in their freezing diluents, can also obviously improve their sperm livability rate(0.35), but not affect their abnormal rate and the apical body rate. 【Conclusion】Adding appropriate concentrations of vitamin E to the frozen semen diluent, can slow the freezing of sperm damage and improve small-tailed Han sheep frozen sperm viability, improve the quality of frozen semen; When adding caffeine dilution of 5mg/ml solution, after frozeing, the motility rate was significantly increased, but does not affect the sperm deformity rate and the rate of acrosome intactness.