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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (28): 105-109.doi: 10.11924/j.issn.1000-6850.casb16020108

• 林学 园艺 园林 • 上一篇    下一篇

南美天胡荽无菌快速繁殖技术研究

崔中翌1,刘 芳1,袁有美2,3,唐映红2,3,陈建荣1   

  1. (1长沙学院生物与环境工程系,长沙 410022；2湖南农业大学农学院,长沙 410128；3长沙惟韵生物科技有限公司,长沙 410022）
  • 收稿日期:2016-02-29 修回日期:2016-05-20 接受日期:2016-05-25 出版日期:2016-10-11 发布日期:2016-10-11
  • 通讯作者: 陈建荣
  • 基金资助:
    湖南省科技计划项目“试管合植小植物景观及商品化”(2014CK4018)；长沙市科技计划项目“名贵花卉试管培养及商品化技术研究”(K1205040-31)。

Sterile Rapid Propagation Technique of Hydrocotyle vulgaris L.

Cui Zhongyi1, Liu Fang1, Yuan Youmei2,3, Tang Yinghong2,3, Chen Jianrong1   

  1. (1Biological Engineering and Environmental Sciences of Changsha University, Changsha 410022;2Agriculture of Hunan Agriculture University, Changsha 410128;3Changsha Only Rhyme Biological Technology Company, Changsha 410022)
  • Received:2016-02-29 Revised:2016-05-20 Accepted:2016-05-25 Online:2016-10-11 Published:2016-10-11

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摘要： 旨在研究不同培养条件对南美天胡荽不定芽再生和生根状况的影响并筛选出最适培养条件。以南美天胡荽带腋芽茎段（约1.0 cm）为外植体，进行腋芽诱导、丛生芽增殖及植株再生研究。结果表明生长调节剂种类及质量浓度对南美天胡荽不定芽的出芽时间和再生率以及生根率有明显影响。所做结果分化率高达89%，生根率高达98%。培养条件为光照强度1500 lx，光照时间12 h/d，温度25℃。南美天胡荽诱导不定芽分化的最佳培养基为MS 6-BA 0.12 mg/L NAA 0.3 mg/L；生根的最佳培养基为MS 6-BA 0.3 mg/L NAA 0.15 mg/L。

关键词: 云南大叶种, 云南大叶种, 晒青茶, 化学成分, 普洱茶, 适制性

Abstract: The aims were to study the effects of different culture conditions on regeneration status of adventitious bud and rooting rate of Hydrocotyle vulgaris L., and screen the optimal culture condition. Using auxiliary stems (about 1.0 cm) of Hydrocotyle vulgaris L. as explants, adventitious bud induction, multiple shoots proliferation and plant regeneration were the focal points in the research. The results showed that type and mass concentration of growth regulator had obvious effects on sprouting time and regeneration rate of adventitious bud. The proliferation rate was 89%, and the rooting rate was 98%. The culture condition was illumination intensity 1500 lx, illumination time 12 h/d and culture temperature 25℃. The effective medium for cluster buds-inducing was MS 6-BA 0.12 mg/L NAA 0.3 mg/L. The effective medium for rooting rate was MS 6-BA 0.3 mg/L NAA 0.15 mg/L.